小青龙汤含药血清对内皮素-1诱导的气道平滑肌细胞增殖作用的影响

目的:观察小青龙汤含药血清对内皮素-1(ET-1)诱导的气道平滑肌细胞(ASMC)增殖作用的影响。方法:实验分6组,正常组(10%正常对照组血清)、模型组(ET-1加10%正常对照组血清)、小青龙汤高剂量组(ET-1加10%小青龙汤高剂量组血清)、小青龙汤中剂量(ET-1加10%小青龙汤中剂量组血清)、小青龙汤低剂量组(ET-1加10%小青龙汤低剂量组血清)、地塞米松组(ET-1加10%地塞米松组血清),每组8孔。采用MTT比色法检测ASMC 24h、48h、72h增殖情况。结果:与模型组比较,小青龙汤低剂量组含药血清各期及中剂量组24h、72h对ASMC增殖均有显著性差异(P<0.05);小青龙汤高剂量组各期、中剂量组48h及地塞米松组各期均有极显著性差异(P<0.01)。结论:小青龙汤药物血清能有效抑制ET-1诱导的ASMC增殖作用,可知抑制哮喘大鼠平滑肌增殖是小青龙汤影响哮喘大鼠气道重建的途径之一。

Effects of Small Qinglong Decoction Medicine-Containing Serum on ASMC Proliferation Action Induced by ET-1

Objective: To observe the effects of Small Qinglong Decoction medicine-containing serum on ASMC proliferation action induced by ET-1.Methods: There were six groups in the experiment: normal group(10% normal control serum),model group(ET-1 added 10% normal control serum),Small Qinglong Decoction high dose group(ET-1 added 10% Small Qinglong Decoction high dose serum),Small Qinglong Decoction middle dose group(ET-1 added 10% Small Qinglong Decoction middle dose serum),Small Qinglong Decoction low dose group(ET-1 added 10% Small Qinglong Decoction low dose serum) and Dexamethasone group(ET-1 added 10% Dexamethasone serum),eight slots every group.ASMC proliferation status of 24h,48h and 72h were detected with MTT chromometry.Results: Compared with model group,ASMC proliferation in Small Qinglong Decoction low dose group medicine-containing serum each stage and middle dose group24h and 72h all had significant difference(P<0.05);Small Qinglong Decoction high dose group each stage,middle dose group 48h and Dexamethasone group every stage all had extremely significant difference(P<0.01).Conclusion: Small Qinglong Decoction medicine-containing serum can actively suppress ASMC proliferation action induced by ET-1,to approve that repressing smooth muscle proliferation of asthma rat is one of the approaches of Small Qinglong Decoction effecting air passage reconstruction in asthma rat.