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| 荧光染料SYBR GreenⅠ及EB在定量PCR中敏感性比较 | |
| 引用本文： | 张茜,谢湘芝,经承学.荧光染料SYBR GreenⅠ及EB在定量PCR中敏感性比较[J].郑州大学学报(医学版),2005,40(3):459-461. |
| 作者姓名： | 张茜 谢湘芝 经承学 |
| 作者单位： | 1. 郑州大学第一附属医院儿科,郑州,450052 2. 广西医科大学实验中心,南宁,530021 3. 广西医科大学第一附属医院儿科,南宁,530021 |
| 基金项目： | 广西壮族自治区科技攻关基金资助项目 0143062 |
| 摘 要： | 目的比较荧光染料SYBR Green I及EB在定量PCR诊断中检测DNA的敏感性.方法分别用琼脂糖凝胶电泳SYBR Green I染色及EB染色,检测26例21-三体综合征患者及20例正常人DNA PCR扩增产物,并进行光密度分析比较.结果SYBR Green I染色于24个循环PCR产物带型清楚,可准确定量检测;EB染色则在28个循环才能进行定量分析.结论荧光染料SYBR Green I染色较EB染色测量DNA敏感、快捷,在定量PCR技术中值得推广应用. |
| 关 键 词： | SYBR Green I EB 定量PCR |
| 修稿时间： | 2004年8月9日 |
Sensitivity comparison of SYBR Green I with EB in Q-PCR |
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| ZHANG Qian,XIE Xiangzhi,JING Chengxue.Sensitivity comparison of SYBR Green I with EB in Q-PCR[J].Journal of Zhengzhou University: Med Sci,2005,40(3):459-461. | |
| Authors: | ZHANG Qian XIE Xiangzhi JING Chengxue |
| Institution: | ZHANG Qian 1),XIE Xiangzhi 2),JING Chengxue 3) 1)Department of Pediatrics,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)Medical Scientific Experimental Center,Guangxi Medical University,Nanning 530021 3)Department of Pediatrics,the First Affiliated Hospital,Guangxi Medical University,Nanning 530021 |
| Abstract: | Aim: To compare the sensitivity of SYBR Green I stain with ethidium bromide(EB)stain in quantitative PCR(Q-PCR).Methods: The DNA samples were isolated from 26 cases of Down's syndrome and 20 normal subjects. The DNA amplification products were subjected to agarose gels with SYBR Green I stain and EB stain, respectively. The optical densitometry of the PCR products was measured.Results: DNA amplification products in 24 cycles stained with SYBR Green I were clearly visualized and quantitatively detected,but DNA amplification products stained with EB could not be quantitatively analyzed until 28 cycles.Conclusion: SYBR Green I is more sensitive and rapid for detecting DNA in Q-PCR assay compared with EB. |
| Keywords: | SYBR Green I EB Q-PCR |
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