| 抗吗啡单克隆抗体的制备及其胶体金交联物的初步应用 |
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| 引用本文： | 谢鑫,金伯泉,朱勇,刘雪松,王文学,李笑松,吴汉源. 抗吗啡单克隆抗体的制备及其胶体金交联物的初步应用[J]. 细胞与分子免疫学杂志, 2003, 19(4): 395-396 |
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| 作者姓名： | 谢鑫 金伯泉 朱勇 刘雪松 王文学 李笑松 吴汉源 |
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| 作者单位： | 1. 第四军医大学基础部免疫学教研室,陕西,西安,710032
2. 陕西省公安厅邢警总队,陕西,西安,710016 |
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| 基金项目： | 陕西省科技研究发展基金部分资助 (No .97K1 4 G7) |
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| 摘 要： | 目的：制备吗啡特异性单克隆抗体(mAb)。方法：将吗啡分子与兔血清白蛋白或牛血清白蛋白交联，免疫BALB／c小鼠并检测免疫效价后，应用淋巴细胞杂交瘤技术制备抗吗啡mAb。将纯化mAb与胶体金颗粒交联后利用纸层析技术进行标本检测。结果：成功地制备了3株抗吗啡mAb，利用其中1株抗体制备的胶体金试纸条，检测样品灵敏度达200μg／L。结论：吗啡mAb胶体金试纸条研制成功将为毒品的现场检测打下基础。
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| 关 键 词： | 毒品滥用 单克隆抗体 胶体金 |
| 文章编号： | 1007-8738(2003)04-395-02 |
Preparation of monoclonal antibodies against morphine and preliminary application of its colloidal gold conjugates |
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| Xin Xi,Bo-Quan Jin,Yong Zhu,Xue-Song Liu,Wei-Xue Wang,Xiao-Song Li,Han-Yuan Wu. Preparation of monoclonal antibodies against morphine and preliminary application of its colloidal gold conjugates[J]. Chinese journal of cellular and molecular immunology, 2003, 19(4): 395-396 |
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| Authors: | Xin Xi Bo-Quan Jin Yong Zhu Xue-Song Liu Wei-Xue Wang Xiao-Song Li Han-Yuan Wu |
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| Affiliation: | Department of Immunology, Fourth Military Medical University, Xi'an 710032, China. |
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| Abstract: | AIM: To prepare monoclonal antibodies (mAbs) against morphine. METHODS: Morphine was conjugated to rabbit serum albumin or bovine serum albumin, respectively and the BALB/c mice were immunized with the conjugated. The mAbs against morphine were obtained by hybridoma technique. The urine samples were detected with the paper chromatography based on crosslinked of the purified mAb with colloidal gold. RESULTS: Three mAbs against morphine were obtained and the sensitivity detected by paper chromatography reached to 200 microg/L. CONCLUSION: The successful preparation of the mAb and its colloid gold conjugates may be useful for field check. |
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| Keywords: | drug abuse monoclonal antibody colloidal gold |
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