Osteogenic capacity of human BM‐MSCs,AT‐MSCs and their co‐cultures using HUVECs in FBS and PL supplemented media |
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| Authors: | Jinling Ma Jeroen J J P van den Beucken Sanne K Both Henk‐Jan Prins Marco N Helder Fang Yang John A Jansen |
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| Institution: | 1. Department of Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, the Netherlands;2. Department of Oral and Maxillofacial Surgery, Beijing Stomatological Hospital, Capital Medical University, Beijing, China;3. Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, the Netherlands;4. Department of Oral and Maxillofacial Surgery, VU University Medical Center / Academic Centre for Dentistry Amsterdam, Amsterdam, the Netherlands;5. Department of Orthopedic Surgery, VU University Medical Center, Amsterdam, the Netherlands |
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| Abstract: | Human bone marrow‐derived mesenchymal stem cells (BM‐MSCs) and human adipose tissue‐derived mesenchymal stem cells (AT‐MSCs) are the most frequently used stem cells in tissue engineering. Due to major clinical demands, it is necessary to find an optimally safe and efficient way for large‐scale expansion of these cells. Considering the nutritional source in the culture medium and method, this study aimed to analyze the effects of FBS‐ and PL‐supplemented media on osteogenesis in stem cell mono‐ and co‐cultures with human umbilical vein endothelial cells (HUVECs). Results showed that cell metabolic activity and proliferation increased in PL‐ compared to FBS‐supplemented media in mono‐ and co‐cultures for both BM‐MSCs and AT‐MSCs. In addition, calcium deposition was cell type dependent and decreased for BM‐MSCs but increased for AT‐MSCs in PL‐supplemented medium in both mono‐ and co‐cultures. Based on the effects of co‐cultures, BM‐MSCs/HUVECs enhanced osteogenesis compared to BM‐MSCs monocultures in both FBS‐ and PL‐supplemented media whereas AT‐MSCs/HUVECs showed similar results compared to AT‐MSCs monocultures. Copyright © 2013 John Wiley & Sons, Ltd. |
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| Keywords: | platelet lysate fetal bovine serum bone tissue engineering stem cells co‐culture osteogenesis |
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