| 右旋儿茶素对四氯化碳或半乳糖胺引起原代培养大鼠肝细胞毒性的作用 |
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| 引用本文： | 方瑞英,陆敏,杨宝珠,楼宜嘉,王立明.右旋儿茶素对四氯化碳或半乳糖胺引起原代培养大鼠肝细胞毒性的作用[J].中国医学科学院学报,1992(3). |
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| 作者姓名： | 方瑞英 陆敏 杨宝珠 楼宜嘉 王立明 |
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| 作者单位： | 中国医学科学院浙江分院 杭州
(方瑞英，陆敏，杨宝珠，楼宜嘉)，中国医学科学院浙江分院 杭州(王立明) |
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| 摘 要： | 利用原代培养大鼠肝细胞的方法,研究右旋儿茶素(d-CTC)对四氯化碳(CCl_4)或半乳糖胺(d-GalN)引起肝细胞毒性的作用,并观察该药对肝酶的直接作用。结果表明:d-CTC 0.6～5.0mg/ml可使含10mmol/L CCl_4或5mmol/Ld-GalN肝细胞培养液中的GOT.GPT及LDH活性显著降低,并呈量效关系。d-CTC 2.5～5.0mg/ml可直接抑制LDH及GPT活性,但对GOT无明显影响。其抑酶作用与抗肝毒而降低酶活性比较,有非常显著的差别,提示d-CTC具有较强的抗肝毒作用。
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| 关 键 词： | 右旋儿茶素 四氯化碳 半乳糖胺 肝细胞 |
Effect of d-Catechin on Carbon Tetrachloride- and d-Galactosamine-lnduced Cytotoxicity in Primary Cultured Rat Hepatocytes |
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| Fang Ruiying,.Effect of d-Catechin on Carbon Tetrachloride- and d-Galactosamine-lnduced Cytotoxicity in Primary Cultured Rat Hepatocytes[J].Acta Academiae Medicinae Sinicae,1992(3). |
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| Authors: | Fang Ruiying |
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| Abstract: | The effects of d-catechin (d-CTC) on carbon tetrachloride- and d-galacto-samine (d-GalN)-induced cytotoxicity in primary cultured rat hepatocytes were examined.After 1.5 h preincubation, d-CTC was added at doses of 0.1-5.0mg /ml to culture medium together with 10 mmol/L CCU or 5 mmol/L d-GalN, respectively. GOT, GPT and LDH levels were measured 1.5 h after treatment. The results showed that d-CTC at doses of 0.6 to 5.0 mg/ml could protect the hepatocytes against the toxic effects of CCU and d-GalN. At the higher doses (2.5-5.0 mg/ml), d-CTC showed weak inhibition of LDH and GPT activities, but these did not influence its antihepatotoxic activity. |
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| Keywords: | d-catechin carbon tetrachloride d-galactosamine hepatocytes |
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