氯化钆对急性坏死性胰腺炎肺损伤的防护作用

目的　探讨氯化钆 (GdCl3 )在急性坏死性胰腺炎 (ANP)肺损伤中的作用。方法　将 90只成年SD大鼠随机分为正常对照组、ANP组、GdCl3 处理组、ANPGdCl3 预处理组、ANPGdCl3 治疗组。每组 18只。逆行性胰胆管注射 3%牛磺酸钠建立ANP大鼠模型。经支气管肺泡灌洗获取肺泡巨噬细胞 (AM ) ,行血气分析 ,检测肺湿 /干重比值、支气管肺泡灌洗液 (BALF)中蛋白含量、肺组织髓过氧化物酶 (MPO)水平、AM分泌肿瘤坏死因子α(TNFα)、一氧化氮 (NO)水平。以琼脂糖凝胶电泳分析、透射电镜观察和流式细胞仪碘化丙啶 (PI)单染色法检测AM凋亡情况。并行肺组织病理学检查。结果　GdCl3 处理组各指标与正常对照组相比差异无显著意义 (P >0 0 5 )。ANPGdCl3 预处理组和ANPGdCl3 治疗组各项指标均升高 ,与正常对照组相比差异有显著意义 (P <0 0 5 ) ,但与ANP组相比升高不明显 ,两组相比差异也有显著意义 (P <0 0 5 )。ANPGdCl3 预处理组和ANPGdCl3 治疗组AM琼脂糖凝胶电泳均可见DNA梯状条带 ,透射电镜观察可见典型凋亡形态学特征 ,流式细胞仪DNA直方图可见G1峰左侧出现典型亚二倍体细胞群峰型 ,而另 3组则均未出现。结论　GdCl3可能通过诱导ANP大鼠AM发生凋亡来减轻ANP所致的肺损伤

The protective role of gadolinium chloride in lung injury associated with acute necrotizing pancreatitis

OBJECTIVE: To discuss the role of gadolinium chloride (GdCl(3)) in lung injury associated with acute necrotizing pancreatitis (ANP). METHODS: Experimental animals were randomized into five groups (n = 18 for each group): normal control group, ANP group, GdCl(3) pretreatment group, ANP GdCl(3) pretreatment group, ANP GdCl(3) treatment group. Rat ANP model was induced by intraductal administration of 3% sodium taurocholate. Alveolar macrophages (AM) were obtained by bronchoalveolar lavage. The blood gas assay, the ratio of wet/dry tissue, protein content of bronchoalveolar lavage fluids (BALF), the myeloperoxidase (MPO) of lung tissue and generation of TNFalpha and NO by AM were evaluated. The apoptosis of AM was checked by agarose gel electrophoresis analysis, transmission electric microscopy observation and cytometry propidium iodide single stained method. The lung tissue was examined by histology. RESULTS: The parameter of GdCl(3) pretreatment group compared with normal control group had no statistical significance (P > 0.05). The indicators of ANP GdCl(3) pretreatment group and ANP GdCl(3) treatment group were elevated compared with the normal control group and had statistical significance (P < 0.05). But compared to the ANP group, they were all decreased and also had the statistical significance (P < 0.05). The 180 - 200 bp ladder pattern unique to apoptosis in agarose gel electrophoresis and the apoptotic typical morphologic feature in AM by transmission electric microscopy and typical subdiploid peak in DNA content figure could be observed in ANP GdCl(3) pretreatment group and ANP GdCl(3) treatment group, while the other three groups could not. CONCLUSIONS: Lung injury associated with ANP could be ameliorated by application of GdCl(3) through inducing apoptosis of AM of ANP.