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SAMP10鼠脑衰老相关基因HSP86、HSP84的表达及针刺影响的研究
引用本文:付于,于建春,丁晓蓉,韩景献.SAMP10鼠脑衰老相关基因HSP86、HSP84的表达及针刺影响的研究[J].中国针灸,2006,26(4):283-286.
作者姓名:付于  于建春  丁晓蓉  韩景献
作者单位:天津中医学院第一附属医院老年病研究室,天津,300193
基金项目:中国科学院资助项目;天津市科技发展项目;天津市卫生局资助项目;天津市高等学校科技发展基金
摘    要:目的:探讨针刺延缓衰老的作用机制。方法:采用快速老化小鼠SAMP10、SAMR1为模型,运用RT-PCR和地高辛标记的非放射性Northern blot技术,观察8月龄SAMR1对照组、SAMP10对照组、SAMP10针刺组及SAMP10非穴位刺激组全脑、皮层和海马HSP84、HSP86基因表达的变化。结果:SAMP10对照组小鼠热休克蛋白HSP84、HSP86在全脑、皮层、海马中的表达下调,针刺后表达上调并趋向于正常组。结论:SAMP10小鼠脑衰老与热休克蛋白HSP84、HSP86基因表达异常有关,针刺可以通过调节HSP84、HSP86基因表达增强细胞保护、抑制细胞凋亡、抵抗氧化应激,从而起到延缓衰老的作用。

关 键 词:衰老/代谢  衰老/针灸效应  基因表达  脑/代谢  脑/针灸效应
文章编号:0255-2930(2006)04-0283-04
修稿时间:2005年6月10日

Study on expression of brain aging-relative genes HSP86 and HSP84 and effects of acupuncture in the SAMP10 mouse
FU Yu,YU Jian-chun,DING Xiao-rong,HAN Jing-xian.Study on expression of brain aging-relative genes HSP86 and HSP84 and effects of acupuncture in the SAMP10 mouse[J].Chinese Acupuncture & Moxibustion,2006,26(4):283-286.
Authors:FU Yu  YU Jian-chun  DING Xiao-rong  HAN Jing-xian
Institution:First Affiliated Hospital of Tianjin College of TCM, Tianjin 300193, China.
Abstract:OBJECTIVE: To probe the mechanism of acupuncture for anti-aging. METHODS: In the senescence accelerated mouse the SAMP10 and the SAMR1, by using RT-PCR and DIG-labeled Northern blot technique, the expression differences of HSP84 and HSP86 genes in whole brain, cortex and hippocampus in the 4 groups,8-month SAMR1 control group, 8-month SAMP10 control group, 8-month SAMP10 acupuncture group and 8-month SAMP10 non-point acupuncture group were investigated. RESULTS: In the SAMP10 control group, the expression of HSP84 and HSP86 were down-regulated in the whole brain, the cortex and the hippocampus, and they were up-regulated after acupuncture, tending to the normal group. CONCLUSION: Brain aging of the SAMP10 mouse is related with abnormal expression of HSP84 and HSP86 genes, and acupuncture can strengthen the protection of cells, inhibit apoptosis and anti-oxidative stress through regulating expression of HSP84 and HSP86, hence anti-aging.
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