新疆汉族、维吾尔族HPV16E6基因变异与子宫颈癌及宫颈病变的关系

目的探讨新疆地区汉族、维吾尔族宫颈癌及宫颈病变中HPV16型E6基因变异分布及频率,比较两个民族之间的差异。方法对140例HPV16阳性标本,设计E6基因特异性引物,通过PCR扩增HPV16E6全长基因,PCR产物直接测序,进行序列分析并与德国标准株对比,筛选突变位点。结果 123例汉族、维吾尔族妇女宫颈癌及宫颈病变标本中均存在HPV16E6变异株,其中突变率分别为:47.37%(27/57)和50%(33/66),E6突变位点主要是L83V、D25E、D64E、I73V、H78Y、D113E,其中L83V变异株在两个民族突变中占的比例均最高,汉族L83V突变频率为29.82%(17/57)显著低于维吾尔族的40.90%(27/66)(P0.05)。而汉族D25E突变频率为19.30%(11/57)显著高于维吾尔族的7.58%(5/66)(P0.05),D64E变异株在维族突变株中占的比例为6.1%,而汉族标本中未检出。结论两个民族HPV16 E6基因变异发生的位置和变异发生的频率存在差异,维吾尔族人群中HPV16E6基因D64E变异株可能与该地区维吾尔族宫颈癌高发存在一定的关系。

The relationship of HPV16E6 mutation and Cervical lesions and cervical carcinoma in Han and Uygur in Xinjiang

Objective To investigate distribution of HPV16E6 mutation and whether there are differences in Han, Uygur cervical cancer(CC) and precancerous lesions.the relationship between the mutation and high incidence of CC in Han, Uygur women. Methods 140case precancerous lesions and cervical carcinoma patients HPV16 genotypes was chosen and HPV16E6 gene was amplified by polymerase chain reaction (PCR). The PCR fragments were sequenced and analyzed. Results There was mutations in 123 cases , mutation rate distribution in Han and Uygur were 47.37%（27/57）and 50%( 33/66) respectively . They were detected mutations D25E、D64E、I73V、H78Y、D113E, L83V, the hot mutation was L83V , rates was 29.82%（17/57）in Han significant lower than 40.90%(27/66) in Uygur, Rates was D25E 19.30%（11/57）in Han significant higher than7.58%(5/66) in Uygur . Rates was D64E 6.1% in Uygur , but have no prevented in Han . Conclusion There was difference between HPV16E6 gene mutation sites and rates in two nations .Our research suggested that the distribution of D64E in Uygur might be associated with high incidence of CC in this nation.