白色紫锥菊不定根诱导及咖啡酸衍生物积累研究

以白色紫锥菊试管苗子叶为外植体,研究了植物生长素2,4-D,IAA,IBA,NAA对不定根诱导以及IBA浓度对液体悬浮培养中不定根的生长及咖啡酸衍生物积累的影响,并进行了生物反应器培养.结果表明,对白色紫锥不定根诱导最适合植物生长素是IBA1.0mg· L-1,不定根诱导数目达到22.5根/培养皿.液体悬浮培养中IBA 1.0 mg·L-1最适合不定根生长及咖啡酸衍生物的积累.白色紫锥菊不定根在5L气升式生物反应器中培养30 d后可获得8.98 g· L-1干重,是三角瓶悬浮培养干重4.38 g·L-1的2.05倍；生物反应器培养的不定根中紫锥菊苷质量分数为14.08 mg·g-1(干重),是栽培根的2.4倍；氯原酸,菊苣酸,总咖啡酸衍生物含量是栽培根的4.0 ～25.6倍.该研究为大量生产紫锥菊药品可提供富含紫锥菊苷等咖啡酸衍生物的高品质生物医学药材.

Induction of adventitious roots of Echinacea pallida and accumulation of caffeic acid derivatives

Objective: To investigate the effect of auxins 2,4-D,IAA,IBA,NAA on induction of adventitious roots as well as that of IBA concentrations on the growth of adventitious roots and the accumulation of caffeic acid derivatives,with test-tube seedling leaves Echinacea pallida as the explant,and cultivate adventitious roots in bioreactors. Result: 1.0 mg·L^-1 IBA was found the best for the induction of adventitious roots,with the numer of induced adventitious roots up to 22.5 in each culture dish. Among different concentrations for suspension cultivation of IBA tested,1.0 mg·L^-1 IBA was found the most suitable for the growth of adventitious roots and the accumulation of caffeic acid derivatives. In a 5 L balloon type bubble bioreactor,8.98 g·L^-1 dry weight was achieved after one month,which was 2.05 times of 4.38 g·L^-1 dry weight cultivated in a triangular flask. The content of echinacoside cultivated in a bioreactor was 14.08 mg·g^-1 DW,which was 2.4 times of cultivated roots. The contents of chlorogenic acid,chicoric acid and total caffeic acid derivatives were 4.0-25.6 times of ultivated roots. Conclusion: The study can provide high-quality biomedical drugs containing such caffeic acid derivatives as echinacoside for mass production of Echinacea purpurea medicines.