| 蛇足石杉内生真菌抗乙酰胆碱酯酶活性筛选及分类鉴定 |
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| 引用本文： | 王莉莉,吕会芳,张丽,化海霞,王杰华,胡之璧,黎万奎.蛇足石杉内生真菌抗乙酰胆碱酯酶活性筛选及分类鉴定[J].中国中药杂志,2012,37(24):3701-3705. |
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| 作者姓名： | 王莉莉 吕会芳 张丽 化海霞 王杰华 胡之璧 黎万奎 |
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| 作者单位： | 上海中医药大学中药研究所中药标准化教育部重点实验室,上海201213;上海中医药大学中药研究所中药新资源与质量标准综合评价国家中医药管理局重点研究室,上海201213 |
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| 基金项目： | 国家自然科学基金项目(30873386,81130070);上海高校优青基金项目(szy10036) |
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| 摘 要： | 目的:从蛇足石杉Huperzia serrata(Thunb.ex Murray) Trevis.内生真菌中筛选具有抗乙酰胆碱酯酶活性的菌株.方法:利用乙酰胆碱酯酶水解α-萘乙酯的产物和固蓝B盐发生显色反应的原理,用薄层色谱-生物自显影法对59株蛇足石杉内生真菌发酵产物进行抗乙酰胆碱酯酶活性筛选,并通过18s rDNA和5.8s rDNA序列分析结合形态学特征对目标菌株进行分类鉴定.结果:蛇足石杉内生真菌LQ2F01在抗乙酰胆碱脂酶活性筛选中呈现阳性颜色反应,18s rDNA和5.8s rDNA序列分析并结合形态学分类表明该菌株属枝顶孢霉属Acremonium.结论:蛇足石杉内生真菌LQ2F01表现出和宿主植物相同的抗乙酰胆碱脂酶活性,在天然药物开发以及内生真菌与宿主植物关系的研究中具有重要意义.
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| 关 键 词： | 蛇足石杉 内生真菌 乙酰胆碱酯酶抑制剂 18s rDNA 5.8s rDNA 枝顶孢霉属 |
| 收稿时间： | 2012/9/20 0:00:00 |
Screening of endophytic fungi from Huperzia serrata for acetylcholinesterase inhibitory activity and its taxonomic identification |
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| WANG Li-li,LV Hui-fang,ZHANG Li,HUA Hai-xi,WANG Jie-hu,HU Zhi-bi and LI Wan-kui.Screening of endophytic fungi from Huperzia serrata for acetylcholinesterase inhibitory activity and its taxonomic identification[J].China Journal of Chinese Materia Medica,2012,37(24):3701-3705. |
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| Authors: | WANG Li-li LV Hui-fang ZHANG Li HUA Hai-xi WANG Jie-hu HU Zhi-bi and LI Wan-kui |
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| Institution: | The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| Abstract: | |
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| Keywords: | Huperzia serrata endophytic fungi acetylcholinesterase inhibitor 18s rDNA 5 8s rDNA Acremonium |
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