HPLC-ELSD法测定牛黄降压丸中黄芪甲苷的含量

目的 建立高效液相色谱—蒸发光散射检测法（HPLC-ELSD法）测定牛黄降压丸中黄芪甲苷的含量.方法 采用安捷伦公司ZORBAX Eclipse XDB-C18色谱柱（4.6 mm×150 mm,5μm）;流动相：乙腈-水（35∶65）,流速：1 mL·min-1;柱温：35℃;检测器：蒸发光散射检测器（Varian 385-LC）.结果 黄芪甲苷在0.1 ～10 μg范围内具有良好的线性关系,回收率大蜜丸为100.3％（RSD =0.9％,n=9）,小蜜丸为99.7％（RSD =0.9％,n=9）.结论 该法具有良好的精密度和重现性,结果准确可靠,可作为本产品的质量控制方法.

Determination of astragaloside IV in Niuhuang Jiangya Pills by HPLC-ELSD

Abstract：Objective To establish an HPLC-ELSD method for determining astragaloside IV in Niuhuang Jiangya Pills. Methods The HPLC- ELSD with the ZORBAX Eclipse XDB- C18 （ 4.6 mm × 250 mm,5 μm ） was used. Acetonitrile-water （35：65） at a flow rate of 1.0 mL · min^- 1 as mobile phase and the temperature of column at 30℃, the detector was ELSD. Results There was a good linear correlation in the range of 0.1 - 10 p,g. The recoveries were 100.3% （ RSD = 0. 9%, n = 9 ） for big honeyed pills, and 99.7% （ RSD = 0. 9%, n = 9 ）for small honeyed pills. Conclusions The method is reliable, accurate and repeatable, which can be applied to the determination of astragaloside IV in Niuhuang Jiangya Pills.