miR-181c-5p靶向TRIM71调控非小细胞肺癌细胞A549的凋亡研究

目的 探讨miR-181c-5p调控非小细胞肺癌细胞A549的潜在机制.方法 检测非小细胞肺癌细胞A549和人正常肺细胞HLF-a中miR-181c-5p的表达水平.过表达或敲低miR-181c-5p后,检测非小细胞肺癌细胞A549的凋亡水平.通过miRDB在线分析miR-181c-5p潜在底物,过表达miR-181c...

Study on miR-181c-5p Targeting TRIM71 to Regulate the Apoptosis of Non-small Cell Lung Cancer Cell A549

Objective To explore the potential mechanism of miR-181c-5p in regulating non-small cell lung cancer cell A549.Methods To detect the expression level of miR-181c-5p in non-small cell lung cancer cell A549 and human normal lung cell HLF-a.After overexpression or knockdown of miR-181c-5p,the apoptosis level of non-small cell lung cancer cell A549 was detected.The potential substrates of miR-181c-5p were analyzed online by miRDB.After miR-181c-5p was overexpressed,the expression level of potential substrates was detected by real-time fluorescent quantitative PCR.After knocking down the potential substrate,the apoptosis level of non-small cell lung cancer cell A549 was detected.Results The expression level of miR-181c-5p in non-small cell lung cancer cell A549 was lower than that of human normal lung cell HLF-a(P<0.05).After overexpression of miR-181c-5p,the apoptosis of non-small cell lung cancer cell A549 increased(P<0.05);after knocking down miR-181c-5p,the apoptosis of non-small cell lung cancer cell A549 decreased(P<0.05).When TRIM71 was knocked down,the apoptosis level of non-small cell lung cancer cell A549 increased(P<0.05).After overexpression of TRIM71,the apoptotic level of non-small cell lung cancer cell A549 decreased(P<0.05).After overexpression of miR-181c-5p,the protein level of TRIM71 decreased;after knocking down miR-181c-5p,the protein level of TRIM71 increased.After knocking down TRIM71,the p53 level of non-small cell lung cancer cell A549 increased;and after overexpression of TRIM71,the p53 level of non-small cell lung cancer cell A549 decreased.After overexpression of miR-181c-5p,the p53 level of non-small cell lung cancer cell A549 increased;and after knocking down miR-181c-5p,the p53 level of non-small cell lung cancer cell A549 decreased.Conclusion miR-181c-5p reduces the expression level of TRIM71 by targeting the 3 terminal non-coding region of TRIM71 mRNA,and then increases the protein level of the apoptosis-activating gene p53,and finally promotes the apoptosis of non-small cell lung cancer cell A549.