| TUNEL法与MTT法检测脑胶质瘤细胞药物敏感性的比较 |
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| 引用本文： | 王静,王秋波.TUNEL法与MTT法检测脑胶质瘤细胞药物敏感性的比较[J].青岛大学医学院学报,2001,37(1):47-49. |
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| 作者姓名： | 王静 王秋波 |
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| 作者单位： | 青岛大学医学院免疫学教研室 |
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| 摘 要： | （1）目的 比较检测脑胶质瘤细胞药物敏感性的两种方法。（2）方法 同时采用DNA断端末端标记（TUNEL）法与噻唑蓝（MTT）法，体外检测人多形性胶质母细胞瘤BT325株细胞TUNEL法和MTT法所得结果高度一致；而用两种方法检测19例病人新鲜脑胶质瘤细胞药物敏感性，实验结果有一定的差异。TUNEL法优于MTT法，（4）结论 在细胞高度均一时，TUNEL法和MTT法均可较好反映药敏情况，但随着所分离的肿瘤细胞纯度降低，MTT法难以准确反映药敏试验结果。
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| 关 键 词： | 脑神经胶质瘤 细胞凋亡 噻唑类 药物筛选试验 抗肿瘤 TUNEL法 MTT法 |
| 文章编号： | 1001-4047(2001)01-0047-03 |
| 修稿时间： | 2000年10月30 |
THE COMPARISON OF TUNEL AND MTT ASSAY FOR DETECTION OF SENSITIVITY OF BRAIN GLIOMA CELLS TO CHEMOTHERAPEUTIC AGENTS |
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| WANG Jing,WANG Qiubo.THE COMPARISON OF TUNEL AND MTT ASSAY FOR DETECTION OF SENSITIVITY OF BRAIN GLIOMA CELLS TO CHEMOTHERAPEUTIC AGENTS[J].Acta Academiae Medicinae Qingdao Universitatis,2001,37(1):47-49. |
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| Authors: | WANG Jing WANG Qiubo |
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| Institution: | WANG Jing,WANG Qiubo\ Department of Immunology,Qingdao University Medical College,Qingdao 266021[ |
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| Abstract: | Objective\ To compare and analyse the two methods,TUNEL and MTT, for detection of chemosensitivity to chemotherapeutic agents in human brain glioma cell and the tumor cell line BT325.\ Methods\ The TUNEL and MTT were both adopted to detect chemosensitivity of the brain glioma cells in 19 patients with brain glioma and the tumor cell line BT325 to eight chemotherapeutic drugs.\ Results\ The chemosensitivity was no difference by using TUNEL and MTT in the tumor cell line BT325. But the chemosensitivity was different by using TUNEL and MTT in 19 patients with brain glioma. The results showed that TUNEL was better than MTT.\ Conclusion\ It would be better to use TUNEL than MTT when the samples were mixed with non tumor cells. |
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| Keywords: | glioma cell death thiazoles drug screening assays antitumor |
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