Phenolic composition and biological activities of Juniperus drupacea Labill. berries from Turkey |
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Authors: | N Miceli A TrovatoA Marino V BellinghieriA Melchini P Dugo F Cacciola P Donato L Mondello A GüvençR De Pasquale MF Taviano |
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Institution: | a Pharmaco-Biological Department, University of Messina, Vill. SS. Annunziata, 98168 Messina, Italy b Plant Natural Products and Health Department, Institute of Food Research, Norwich Research Park, NR47LX Norwich, United Kingdom c Pharmaco-Chemical Department, University of Messina, Vill. SS. Annunziata, 98168 Messina, Italy d Campus Bio-Medico University, Via Alvaro del Portillo 21, 00128 Roma, Italy e Chromaleont s.r.l A spin-off of the University of Messina, Via Industriale, 143, 98123 Messina, Italy f Department of Pharmaceutical Botany, Ankara University, Tando?an 06100, Ankara, Turkey |
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Abstract: | The present study was designed to define the phenolic profile and the biological potential of berries methanol extract of Juniperus drupacea Labill. from Turkey.The total phenolic content (Folin-Ciocalteau assay) was 48.06 ± 0.99 mg GAE/g extract. The HPLC-DAD-ESI-MS analysis allowed the determination of the complete phenolic profile of J. drupacea berries. Phenolic acids represented more than 60% of the total phenolics, and tyrosol was the major one (1324 ± 0.64 μg/g extract); within the flavonoids amentoflavone was detected as the main constituent (927 ± 0.35 μg/g extract).The extract exhibited good antioxidant properties, as determined by different in vitro models: DPPH test (IC50 0.38 ± 0.02 mg/mL), reducing power (12.63 ± 0.14 ASE/mL), Fe2+ chelating ability (IC50 2.26 ± 0.06 mg/mL), and TBA test (IC50 2.47 ± 1.13 μg/mL).Cytotoxicity against Artemia salina was highlighted (LC50 489.47 ± 27.8 μg/mL), and a significant decrease (p ? 0.05; p ? 0.01) in HepG2 cells viability was observed at the higher concentrations (5-10 μg/mL).The extract displayed good antibacterial activity towards Gram-positive bacteria and in particular Staphylococcus aureus was the most susceptible strain (MIC 78.12 μg/mL). |
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Keywords: | Juniperus drupacea HPLC-DAD-ESI-MS Phenolic compounds Antioxidant activity Antimicrobial activity Cytotoxic activity |
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