中国恒河猴(Macaca mulatta)外周血Th17细胞及其在SHIV感染后的变化

目的: 对正常中国恒河猴(Macaca mulatta)及嵌合猿猴/人免疫缺陷病毒(SHIV)感染过的中国恒河猴外周血中CD4 IL-17A 和CD4-IL-17A T淋巴细胞的分布频率进行初步观察.方法: 用荧光染料标记的单克隆抗体(mAb)对10只中国恒河猴的外周血或PMA Ionomycin刺激后的PBMC细胞表面的CD3、 CD4、 CD8及细胞内IL-17A进行免疫荧光染色.用FACScalibaur获取染色后的样品, 所得数据用CellQuest进行分析.结果: 在6只正常中国恒河猴个体中均能检测到IL-17A 淋巴细胞, 未经刺激培养的样品中IL-17A 细胞频率很低, 但在短期刺激培养后淋巴细胞中具有约1.4%的淋巴细胞为IL-17A 细胞, 明显多于刺激前的IL-17A 淋巴细胞; 在短期刺激培养后CD3 CD4 淋巴细胞中大约有2.7%的IL-17A 细胞或CD4 Th17细胞.对4只SHIV感染个体中Th17细胞的初步分析未发现它们与正常个体间的统计学差异.结论: 与人类相似, 中国恒河猴的外周血中也存在一定比例的Th17细胞, 为进一步利用恒河猴AIDS动物模型分析HIV/AIDS与Th17细胞的关系提供了基础.

Th17 lymphocytes in the peripheral blood of Chinese rhesus macaques(Macaca mulatta) with or without SHIV infection

AIM: To observe CD4(+)IL-17(+) cells in the peripheral blood of Chinese rhesus macaques. METHODS: Peripheral blood samples were collected from normal and SHIV infected rhesus macaques. Surface CD3, CD4, CD8 and intracellular IL-17A expression of peripheral blood (with or without PMA+Ionomycin stimulation) were detected by immunostaining with fluorochrome labeled monoclonal antibodies. The samples were acquired with FACScalibur and the data were analyzed with CellQuest. RESULTS: IL-17A(+) cells were observed in all the 6 normal rhesus macaques but there were very few before cultured with PMA+Ionomycin. About 1.4% of lymphocytes were IL-17A(+) cells after stimulation and short-term cultivation, and about 2.7% of CD3(+)CD4(+) lymphocytes were IL-17A(+) cells, i.e. CD4(+) Th17 cells. Th17 cells in the 4 SHIV infected rhesus monkeys were also examined. The number of the Th17 cells detected after stimulation and short-term cultivation was not significantly different from that of normal monkeys. CONCLUSION: Similar to human race, Chinese rhesus macaques possess a subset of CD4(+)IL-17A(+) cells, suggesting these rhesus monkey can be used to study the relationship between Th17 cells and HIV/AIDS.