首页 | 本学科首页   官方微博 | 高级检索  
     

内皮素反义基因治疗门静脉高压症的研究
引用本文:毕向军,陈旻湖,陈文红,陈为,王锦辉,朱森林. 内皮素反义基因治疗门静脉高压症的研究[J]. 中华肝胆外科杂志, 2006, 12(4): 250-252
作者姓名:毕向军  陈旻湖  陈文红  陈为  王锦辉  朱森林
作者单位:1. 510010,广州市,广州军区广州总医院干部内二科
2. 510089,广州市,中山大学第一附属医院消化内科
基金项目:本研究受2002年广东省自然科学基金重点项目资助(21889)
摘    要:目的研究内皮素-1反义寡核苷酸(ET1-ASON)在内毒素(LPS)致门静脉高压中的作用.方法应用ET1-ASON预处理后将LPS以1 mg/kg体重腹腔注射大鼠,以正义、错义ET1-ASON和生理盐水作为对照,4 h后检测大鼠PVF、PVP、PVR和IVCP;应用放射免疫法、RT-PCR检测各组门静脉血和肝脏的ET1表达情况.结果ET1-ASON处理组大鼠门静脉血浆和肝脏组织ET1表达减低;PVR和PVP较对照组明显降低,PVF无明显改变.结论ET1-ASON在内毒素致门脉高压中主要通过降低PVR起作用.

关 键 词:高血压  门静脉 内毒素 内皮素-1 反义寡核苷酸
收稿时间:2005-03-23
修稿时间:2005-06-30

Effects of endothelin-1 on portal hypertension induced by endotoxin in vivo
BI Xiangjun, CHEN Minhu, CHEN Wenhong. Effects of endothelin-1 on portal hypertension induced by endotoxin in vivo[J]. Chinese Journal of Hepatobiliary Surgery, 2006, 12(4): 250-252
Authors:BI Xiangjun   CHEN Minhu   CHEN Wenhong
Affiliation:Second Department of Geriatrics, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, P. R. China
Abstract:Objective To investigate the effects of endothelin-1(ET1)on portal hypertension induced by endotoxin.Methods The rats were intraperitoneally injected with different doses of LPS. PVP,IVCP,PVF and PVR were recorded 4 h after the injection.ET1 anti-sense oligonucleotide and cation lipofectamine were mixed and then injected into the portal vein of rats.Twelve hours later,1 mg/kg LPS was injected and PVP,IVCP and PVF were recorded again.The liver and portal blood samples were used to detect ET1 by radioimmunoassay.ET1 mRNA of the liver tissue was detected by RT-PCR.Results The PVF and PVP in the rats were obviously increased with the dose of LPS. PVR decreased and the decrease was not related to the dose of LPS.ET1 antisense oligonucleotide partially counteracted the effects of LPS on portal hemodynamics by decreasing PVR and PVP.LPS prompted the expression of ET1 in liver tissue and serum of the rats.Conclusions ET1 plays an im- portant role in portal hypertension induced by endotoxin through increasing PVR.ET1 antisense oli- gonucleotide can partially inhibit portal hypertension induced by LPS.
Keywords:Hypertension, portal   Endotoxin   Endothelin-1   Antisense oligonucleotide
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号