姬松茸ω-6多不饱和脂肪酸对高血脂鼠的降血脂作用

目的：观察姬松茸ω-6多不饱和脂肪酸（ω-6APFA）对高血脂鼠的降血脂作用。方法：大鼠60只，随机分为空白对照组、模型组、天然大豆磷脂胶囊(NSPC)0.70 g•kg^-1剂量组和ω-6APFA 0.28、0.14、0.07 g•kg^-1 3个剂量组( n=10)。除空白对照组外均连续给予高脂饲料14 d，于第14天起开始连续灌胃给药14 d，对照组和模型组给予等容积蒸馏水10 mL•kg^-1。实验结束后麻醉大鼠，腹主动脉取血测血清总胆固醇(T-CHO)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)。同时测肝脏超氧化物歧化酶(SOD)活性、肝脏丙二醛(MDA)含量及腹腔脂肪蓄积系数。雄性小鼠72只，随机分为空白对照组、模型组、NSPC 1.0 g•kg^-1剂量组、ω-6APFA 0.4、0.2、0.1 g•kg¹ 3个剂量组(n=12)，连续给药7 d，末次给药前16 h除空白对照组，均腹腔注射75％蛋黄生理盐水液0.5 mL，并开始饥饿，末次给药后1 h，于眼球采血，测血清T-CHO及TG含量。结果：与模型组比较，NSPC 0.70 g•kg^-1和ω-6APFA 0.28 g•kg^-1 剂量组大鼠血清T-CHO、TG均明显降低，血清HDL-C升高（P<0.001）；NSPC 0.70 g•kg^-1和ω-6APFA 0.28、0.14 g•kg^-1 剂量组大鼠肝组织SOD活性升高（P<0.05、P<0.001及 P<0.05），MDA降低（P<0.01、P<0.001及 P<0.01），大鼠腹腔脂肪系数均降低（P<0.01）。与模型组比较,NSPC 1.0 g•kg^-1、ω-6APFA 0.4、0.2 g•kg^-1组急性高血脂小鼠T-CHO和TG明显降低（P<0.05、P<0.01及P<0.05）。结论：ω-6APFA对高血脂鼠有明显的降血脂作用。

Action of lowering blood fat of Agaricus blazei Murill ω-6 polyunsaturated fatty acid on hyperlipemia rats and mice

Objective To observe the action of lowering blood fat of Agaricus blazei Murill ω-6 polyunsaturated fatty acid(ω-6APFA) on hyperlipemia rats and mice.Methods Sixty rats were randomly divided into control group,model group,natural soybean phospholipids capsule(NSPC)0.70 g·kg~(-1) group,ω-6APFA 0.28,(0.14,)0.07 g·kg~(-1) groups(n=10).The other groups except control group were given high-fat diets for 14 d,on the fourteenth day the rats were administered orally,the control group and model group were administered distilled water 10 mL·kg~(-1) at the same volume,14 days after continuous administration,rats were anesthetized,the blood were extracted from abdominal artery,T-CHO,TG,HDL-C,LDL-C in sera were determined. At the same time,the activity of SOD in liver and the content of MDA were determined,the fat accumulated coefficient was calculated.72 male mice were randomly divided into control group,model group,NSPC 1.0 g·kg~(-1) group,(ω-6APFA)0.4,0.2,0.1 g·kg~(-1) groups(n=12).Mice were administered continuously,16 h before the last administration,except control group,the mice in the other groups were injected 75% yolk physiological salt solution 0.5 mL through the abdominal cavity,and began to starve,1 h after the last administration,blood was extracted from eyeball,serum T-CHO and TG were determined.Results Compared with model group,T-CHO and TG in rats treated with NSPC 0.70 g·kg~(-1) and ω-6APFA 0.28 g·kg~(-1) all reduced and HDL-C raised obviously(P<0.001),the activities of liver SOD in rats treated with NSPC 0.70g·kg~(-1) and ω-6APFA 0.28,0.14 g·kg~(-1) obviously raised(P<0.05,P<0.001,P<0.05),MDA reduced obviously(P<0.01,P<0.001,P<0.01),fat accumulation coefficient in rats treated with NSPC 0.70 g·kg~(-1) and ω-6APFA reduced obviously((P>0.01).)Compared with model mice,T-CHO and TG in acute hyperlipemia mice treated with NSPC 1.0 g·kg~(-1) and ω-6APFA0.4,0.2 g·kg~(-1) reduced obviously(P<0.05,P<0.01,P<0.05).Conclusion ω-6APFA has the function of lowering blood fat on hyperlipemia rats and mice.