噪声性听力损失易感性与SOD2单核苷酸多态性的关联

目的 探讨噪声性听力损失(NIHL)易感性与中国汉族人群SOD2 rs2842980、rs5746136、rs2758331、rs4880和rs5746092单核苷酸多态性之间的关联.方法 利用病例对照研究,通过比较同一噪声接触强度作业人员的左耳3000 Hz频段听阈位移情况,筛选出听阈位移最大的10%个体作为易感人群组,共201例,听阈位移最小的10%个体作为耐受人群组,共202例,并进行相关的职业卫生调查和问卷调查.抽取易感人群和耐受人群空腹外周静脉血5 ml用于Qiagen试剂盒抽提基因组DNA,TaqMan探针法化学荧光等位基因鉴别试验检测SNP.结果 SOD2基因SNP rs4880 C等位基因是NIHL的危险因素,与T等位基因相比,OR值为1.76,95%CI为1.17～2.63,P=0.006.携带CC和CT基因型的个体患NIHL的风险是TT基因型的2.24倍,95%C/为1.43-3.50,校正混杂因素后仍可发现CC和CT基因型是NIHL的保护因素,与TT基因型相比,OR(95%CI)为2.45(1.51～3.96).结论 在中国汉族人群中,噪声性听力损失易感性与SOD2线粒体靶向定位序列的rs4880单核苷酸多态性有关.

Association between SNPs in SOD2 and noise-induced hearing loss

Objective To observe the association between SNPs in SOD2 and noise-induced heating loss in Chinese Han population. Methods A case-control study was designed to study the effect of environ-menial risk factors on susceptibility to NIHL in 201 sensitive workers and 202 resistant workers. A question-naire was designed to carry out an investigation, and an occupational health survey was used to identify the oc-cupational risk factors. Genomic DNA was extracted from peripheral blood cells samples using standard proce-dures of Qiagen kit. SNPs were detected using standard procedures of TaqMan probe allele identification method. Results In SOD2 gene, the C allele of rs4880 was a risk factor of NIHL compared with the T allele,OR =1.76, 95%CI 1.17～2.63, P=0.006. CC and CT genotypes compared with TT were risk factors, crude OR =2.24, 95% CI 1.43～3.50 and adjusted OR = 2.45, 95% CI 1.51～3.96, P<0.001, respectively. Conclusion In Chinese Han population, SOD2 rs4880 SNP in the mitochondrial targeting sequence is associated with noise induced hearing loss.