密骨方对去卵巢大鼠骨折的影响

目的 采用卵巢切除法建立原发性骨质疏松及骨折动物模型,观察中药密骨方对去势大鼠骨痂组织的影响.方法 将90只雌性Wistar大鼠随机分为对照组(sham),模型组(OVX)与密骨方治疗组(MGF),模型组及治疗组行卵巢摘除术,同时3组大鼠均致其右侧股骨上1/3处骨折.MGF组按每日100mg/kg体重的剂量灌胃给药,Sham组和OVX组给予同体积的生理盐水,每日1次,疗程分别为2、4、6周,用SABC免疫组织化学方法检测骨折端转化生长因子(TGF-β1)的表达.结果 在骨折端MGF组软骨细胞的阳性表达率较高;TGF-β1的表达在时间的变化上呈显著升高趋势;第4、6周TGF-β1表达显著高于OVX组和Sham组.结论 密骨方对骨质疏松性骨折的愈合有一定的促进作用.

Effects of Migu decoction on the callus and fracture of ovariectomized rats

Objective To establish animal models of primary osteoporosis and fracture animal models and observe the effects of Migu decoction on the callus of ovariectomized (OVX) rats. Methods Ninety Wistar female rats were randomly divided into 3 groups:the sham-ovariectomized (sham) ,the OVX and the Migu decoction (MGD) groups. The rats in OVX and the MGD groups were ovariectomized. The rats in all groups were artificially fractured on the upper part of the right femur. The rats in MGD group OVX groups were administrated with the same volume of normal sodium (NS) once daily by stomach ga-rage. The treatment duction was 2,4 and 8 weeks respectively. The expression of transforming growth fac-tor- β1 (TGF-β1) in fracture ends was detected by SABC immunohistochemistry staining. Results The positive expression rate of TGF-β1 in MGD group was high in the fracture ends. The expression of TGF-β1 was increased with the prolongation of time. On the 4th and 6th week,the expression of TGF-β1 was signif-icantly higher than in OVX and sham groups. Conclusion MG decoction can promote the healing of oste-oporosis-induced fracture to some extent.