醋酸曲安缩松及丝裂霉素对瘢痕成纤维细胞整联蛋白表达的影响

目的：了解醋酸曲安缩松、丝裂霉素对瘢痕成纤维细胞整联蛋白表达的影响，初步探讨两种药物抗瘢痕增生的作用机制。方法：体外培养瘢痕组织成纤维细胞，利用β１，α１～４整联蛋白单克隆抗体，通过原位ＥＬＩＳＡ技术检测瘢痕成纤维细胞在不同质量浓度醋酸曲安缩松（１，１０，５０，１００μｇ／ｍｌ）和丝裂霉素（１，５，１０，２０μｇ／ｍｌ）作用下整联蛋白的表达水平。结果：醋酸曲安缩松、丝裂霉素均能不同程度地抑制瘢痕成纤维细胞整联蛋白的表达，抑制效果在实验范围内随药物浓度的增加而提高。其中醋酸曲安缩松对β１，α１～４整联蛋白表达的最大有效抑制浓度（μｇ／ｍｌ）分别为５０，１００，１００，５０，１００，抑制率分别达１６．４４％，３４．７４％，１５．９２％，３７．９５％和３２．１７％；丝裂霉素对它们的最大有效抑制浓度（μｇ／ｍｌ）分别为

Effect of triamcinolone acetonide and mitomycin on integrin expressions in hypertrophic scar-derived fibroblasts

Objective:To investigate the effect of triamcinolone acetonideand mitomycin on expression levels of β1 and α1-4 integrins in hypertrophic scar-derived fibroblasts,and study the molecular mechanism of above drugs in the treatment of hypertrophic scar.Methods:Hypertrophic scar-derived fibroblasts were cultured in the presence of various concentration triamcinolone acetonide,and mitomycin.The expression levels of β1 and αl-4 integrins in the cell surface were determined by enzyme-linked immunosorbent assay (ELISA).Results:The shape of cultured hypertrophic scar-derived fibroblast was not changed in the presence of various concentration triamcinolone acetonide and mitomycin. The expression of β1 and αl-4 integrins in hypertrophic scar-derived fibroblasts were inhibited to some extent by triamcinolone acetonide and mitomycin.Conclusion:The results suggest that β1 and αl-4 integrins may be an important intermediary factor on triamcinolone acetonide and mitomycin in the treatment of hypertrophic scars.