| 三羟异黄酮对人乳腺癌耐药细胞mdr-1、HER2/neu表达的影响 |
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| 引用本文： | 王耕,黄韬,薛家鹏,王明华,惠震.三羟异黄酮对人乳腺癌耐药细胞mdr-1、HER2/neu表达的影响[J].华中科技大学学报(医学版),2011,40(5):589-592,610. |
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| 作者姓名： | 王耕 黄韬 薛家鹏 王明华 惠震 |
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| 作者单位： | 1. 华中科技大学同济医学院附属协和医院普外科,武汉,430022
2. 湖北医药学院附属太和医院内分泌血管外科,十堰,442000 |
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| 摘 要： | 目的研究三羟异黄酮(Genistein,GEN)对体外培养人乳腺癌MCF-7/ADM耐药细胞中mdr-1、HER2/neu基因及蛋白表达的影响,探讨其逆转多药耐药机制。方法采用MTT法检测GEN作用MCF-7/ADM细胞后的细胞增殖抑制率(IR)和半数抑制浓度(IC50);RT-PCR法检测MCF-7/ADM细胞经不同浓度阿霉素(ADM)、GEN及GEN联合ADM处理后,其mdr-1、HER2/neu mRNA表达的变化;Western blot检测其对c-erbB2蛋白及P-糖蛋白(P-gp)的影响。结果 GEN对MCF-7/ADM细胞生长抑制作用明显,其抑制效应呈时间-剂量依赖性,特别是GEN浓度增加到60μg/mL时,对细胞抑制作用急剧升高(P<0.01)。MCF-7/ADM细胞中有mdr-1、HER2/neu过量表达,经GEN单独及联合ADM作用后,HER2/neu mRNA表达明显下调,c-erbB2蛋白出现一致性的表达下调,但对mdr-1mRNA及P-gp无影响。结论人乳腺癌MCF-7/ADM细胞耐药性与耐药基因及原癌基因的高表达相关,GEN能下调MCF-7/ADM细胞HER-2/neu基因及蛋白表达,可能是其逆转MCF-7/ADM细胞多药耐药性的机制之一,但对由P-gp介导的多药耐药无效。
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| 关 键 词： | 三羟异黄酮 乳腺癌 MCF-7/ADM 基因 mdr-1 基因 HER2/neu |
Influence of Genistein on mdr-1 and HER2/neu Gene Expression in MCF-7/ADM Cells |
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| Wang Geng ,Huang Tao,Xue Jiapeng et al.Influence of Genistein on mdr-1 and HER2/neu Gene Expression in MCF-7/ADM Cells[J].Journal of Huazhong University of Science and Technology(Health Sciences),2011,40(5):589-592,610. |
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| Authors: | Wang Geng Huang Tao Xue Jiapeng |
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| Institution: | Wang Geng 1,Huang Tao1,Xue Jiapeng2 et al 1Department of General Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China 2Department of Endocrinology and Vascular Surgery,Taihe Hospital Affiliated to Hubei Medical College,Shiyan 442000,China |
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| Abstract: | Objective To study the influence of Genistein(GEN)on multidrug resistance gene-1(mdr-1)and HER2/neu gene and protein expression in MCF-7/ADM cells in vitro,and the reversal mechanism of multidrug resistance. Methods MTT method was employed to detect the inhibition rate(IR)and 50% inhibition concentration(IC50)of GEN on MCF-7/ADM cells.RT-PCR was employed to detect the influence of GEN on the expression of mdr-1 and HER2/neu in MCF-7/ADM cells.By using Western blot,the effect of GEN on c-erbB2 protein and P-... |
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| Keywords: | Genistein breast cancer MCF-7/ADM multidrug resistance gene-1 HER-2/neu |
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