| 5-氟尿嘧啶联合奥沙利铂对SW480细胞成瘤性及其Galectin-3表达的影响 |
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| 引用本文： | 支国俊,邹游,刘韦成,杨锐,李小兰,陶德定,龚建平,胡俊波,严群. 5-氟尿嘧啶联合奥沙利铂对SW480细胞成瘤性及其Galectin-3表达的影响[J]. 华中科技大学学报(医学版), 2011, 40(5): 585-588. DOI: 10.3870/j.issn.1672-0741.2011.05.021 |
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| 作者姓名： | 支国俊 邹游 刘韦成 杨锐 李小兰 陶德定 龚建平 胡俊波 严群 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院胃肠外科中心,武汉,430030 |
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| 摘 要： | 目的探讨5-氟尿嘧啶(5-Fu)联合奥沙利铂对SW480细胞成瘤性及其对肿瘤中Galectin-3(半乳糖凝集素-3)表达的影响。方法以人结肠癌SW480细胞建立裸鼠移植瘤模型,实验分5-Fu组,奥沙利铂组,5-Fu+奥沙利铂组和注射等体积生理盐水的对照组。采用免疫组织化学方法检测移植瘤中Galectin-3的表达,以图像信号采集与分析系统进行图像扫描分析。结果 5-Fu组、奥沙利铂组和5-Fu+奥沙利铂组抑瘤率分别为30.53%、34.15%和67.22%;免疫组化显示给药组肿瘤组织中Galectin-3表达明显减弱,Galectin-3在联合用药组肿瘤组织中表达(28.875±7.491)较对照组(281.438±45.639)和单独用药组(157.644±33.721,150.682±25.539)明显减弱,图像灰度值统计分析显示差异均具有统计学意义(P<0.05或P<0.01)。结论 5-Fu和奥沙利铂可抑制肿瘤细胞生长,二者联用抑瘤作用增强。肿瘤的生长体积与Galectin-3的表达具有同向性,提示两者之间可能存在直接或间接的联系。
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| 关 键 词： | 5-氟尿嘧啶 奥沙利铂 结肠癌 SW480细胞 半乳糖凝集素-3 |
Effects of 5-Fu Combined with Oxaliplatin on Tumor Formation and Galectin-3 Expression in SW480 Colon Cancer Cells |
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| Zhi Guojun,Zou You,Liu Weicheng et al Center of Gastrointestinal Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan ,China. Effects of 5-Fu Combined with Oxaliplatin on Tumor Formation and Galectin-3 Expression in SW480 Colon Cancer Cells[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2011, 40(5): 585-588. DOI: 10.3870/j.issn.1672-0741.2011.05.021 |
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| Authors: | Zhi Guojun Zou You Liu Weicheng et al Center of Gastrointestinal Surgery Tongji Hospital Tongji Medical College Huazhong University of Science Technology Wuhan China |
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| Affiliation: | Zhi Guojun,Zou You,Liu Weicheng et al Center of Gastrointestinal Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
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| Abstract: | Objective To study the effects of 5-Fu combined with Oxaliplatin on tumorigenesis of SW480 cells and the expression of Galectin-3 in the tumor tissues. Methods Human colon cancer xenografts in nude mice were established by using SW480 cells.5-Fu group,Oxaliplatin group,5-Fu+Oxaliplatin group,and saline control group were set up.By using immunohistochemistry the expression of Galectin-3 was detected in the xenografts.Image signal acquisition and analysis system was applied for image scanning analysis. Result... |
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| Keywords: | 5-Fu Oxaliplatin colon cancer SW480 Galectin-3 |
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