细胞连接对组织冷冻损伤的作用

目的 了解在无保护剂条件下细胞连接在组织冷冻损伤中的作用。方法 采用细胞单层中可形成细胞连接的犬上皮细胞系（MDCK）细胞和不形成细胞连接的中国仓鼠成纤维细胞系（V79）细胞,分为细胞悬液组、贴壁组和单层细胞组分别进行0－40℃之间的梯度冷冻实验,然后采用新型的四氮唑盐法和脂溶性细胞活化剂法测定细胞的活力并进行比较。结果 两种方法测定的结果一致,均显示两种细胞的细胞悬液冷冻后活力高于贴壁组和单层细胞组;MDCK细胞贴壁组活力高于单层细胞组,V79细胞则无明显差异;MDCK单层细胞组活力却明显低于V79单层细胞组。结论 组织细胞间的连接以及细胞与细胞基质的连接对组织冷漠的损伤有重要的作用,它们的存在直接影响组织细胞的活力,其原因可能与构成细胞连接的细胞骨架系统有关。

The role of cell junction in hypothermic impairment of tissue

Objective To investigate the role of cell junction in the hypothermal impairment of tissue without any cryoprotectant. Methods Epithelial MDCK cell line which is able to form well-defined cell junctions after confluency, and fibroblast V79 cell line which is unable to be used for graded freezing experiments,either in suspension, single tough attached to flasks or as monolayers on flasks. Then the viability was assessed by WST-1 and MTT, respectively. Results The results of viability of the two types of cells assessed by WST-1 were well-fitted with that of MTT. The recovery of the two types of cells in suspension was higher than that of single tough group and monolayer group. The single tough group of MDCK had a higher recovery than did the monolayer group. At the same time, such tendency was not observed on V79 cells. As compared with V79 monolayers, lower recovery was found on MDCK monolayers. Conclusions The junction between cells and the adhesion between cell and matrix have important roles in the hypothermic impairment of tissue. Their existence affect the recovery of cells under hypothermia. The cytoskeleton system may contribute to the impairment caused by junctions.