| Comparison of Di-n-methyl Phthalate Biodegradation by Free and Immobilized Microbial Cells |
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| 引用本文： | Wang JL,Ye YC,Wu WZ. Comparison of Di-n-methyl Phthalate Biodegradation by Free and Immobilized Microbial Cells[J]. Biomedical and environmental sciences : BES, 2003, 16(2): 126-132 |
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| 作者姓名： | Wang JL Ye YC Wu WZ |
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| 作者单位： | [1]LaboratoryofEnvironmentalTechnology,InstituteofNuclearEnergyTechnology,TsinghuaUniversity,Beijing100085,China [2]SchoolofEnvironmentScience,PekingUniversity,Beijing100871,China |
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| 基金项目： | the National Natural Science Foundation of China (Grant No. 29637010). |
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| 摘 要： | Objective To compare the biodegradation of di-n-methyl pathalate by free and immobilized microbial cells. Methods The enrichment and isolation technique was used to isolate the microorganism. The PAV-entrapment method was utilized to immobilize the microorganisms. The scanning electron microscophy (SEM) was used to observe the growth and distribution of microbial cells immobilized inside the PVA bead gels. The GC/MS method was used to identify the main intermediates of DMP degradation. Results The microbial cells could grow quite well in PVA gel.The metabolic pathway did not change before and after immobilization of the microbial cells. The degradation rate of immobilized cells was higher than that of flee cells. Conclusion The immobilized microbial cells possess advantages than free cells when applied to the biodegradation of toxic organic pollutants.
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| 关 键 词： | 邻苯二甲酸酯 生物降解 可塑剂 环境污染 Di-n-methyl 微生物 |
Comparison of di-n-methyl phthalate biodegradation by free and immobilized microbial cells |
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| Wang Jian Long,Ye Yu-Cai,Wu Wei-Zhong. Comparison of di-n-methyl phthalate biodegradation by free and immobilized microbial cells[J]. Biomedical and environmental sciences : BES, 2003, 16(2): 126-132 |
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| Authors: | Wang Jian Long Ye Yu-Cai Wu Wei-Zhong |
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| Affiliation: | Laboratory of Environmental Technology, Institute of Nuclear Energy Technology, Tsinghua University, Beijing 100085, China. wangjl@tsinghua.edu.cn |
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| Abstract: | To compare the biodegradation of di-n-methyl pathalate by free and immobilized microbial cells. Methods The enrichment and isolation technique was used to isolate the microorganism. The PAV-entrapment method was utilized to immobilize the microorganisms. The scanning electron microscophy (SEM) was used to observe the growth and distribution of microbial cells immobilized inside the PVA bead gels. The GC/MS method was used to identify the main intermediates of DMP degradation. Results The microbial cells could grow quite well in PVA gel. The metabolic pathway did not change before and after immobilization of the microbial cells. The degradation rate of immobilized cells was higher than that of free cells. Conclusion The immobilized microbial cells possess advantages than free cells when applied to the biodegradation of toxic organic pollutants. |
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| Keywords: | Priority pollutants Phthalic acid ester Immobilized microbial cells Biodegradation |
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