| Effect of Rat Schwann Cell Secretion on Proliferation and Differentiation of Human Neural Stem Cells |
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| 作者姓名： | An YH Wan H Zhang ZS Wang HY Gao ZX Sun MZ Wang ZC |
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| 作者单位： | BeijingNeurosurgicalInstitute,Beijing100050,China |
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| 基金项目： | This research is supported by grants from the Chinese Postdoctoral Foundation and the Beijing Young Scientist Culture Foundation. |
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| 摘 要： | Objective:To investigate the effect of rat Schwann cell secretion on the proliferation and differentiation of human embryonic neural stem cells(NSCs).Methods:The samples were divided into three groups.In Group One,NSCs were cultured in DMED/F 12 in which Schwann cells had grown for one day.In Group Two,NSCs and Schwann cells were co-cultured.In Group Three,NSCs were cultured in DMEM/F12.The morphology of NSCs was checked and β-tubulin,GalC,hoechst 33343 and GFAP labellings were detected.Results:In Group One,all neural spheres were attached to the bottom and differentiated.The majority of them were β-tubulin positive while a few of cells were GFAP or GalC positive.In Group Two,neural Spheres remained undifferentiatied and their proliferation was inhibited in places where Schwann cells were robust.In places where there were few Schwann cells,NSCs performed in a similar manner as in Group One.In group Three,the cell growth state deteriorated day after day,On the 7th day,most NSCs died.Conclusion:The secretion of rat Schwann cells has a growth supportive and differentiation-inducing effect on human NSCs.
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| 关 键 词： | 许旺细胞 细胞增殖 神经中枢细胞 神经生长因子 免疫组织化学 |
Effect of rat Schwann cell secretion on proliferation and differentiation of human neural stem cells |
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| An YH,Wan H,Zhang ZS,Wang HY,Gao ZX,Sun MZ,Wang ZC.Effect of Rat Schwann Cell Secretion on Proliferation and Differentiation of Human Neural Stem Cells[J].Biomedical and Environmental Sciences,2003,16(1):90-94. |
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| Authors: | An Yi-Hua Wan Hong Zhang Ze-Shun Wang Hong-Yun Gao Zhi-Xing Sun Mei-Zhen Wang Zhong-Cheng |
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| Institution: | Beijing Neurosurgical Institute, Beijing 100050, China. riveran@163.com |
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| Abstract: | OBJECTIVE: To investigate the effect of rat Schwann cell secretion on the proliferation and differentiation of human embryonic neural stem cells (NSCs). METHODS: The samples were divided into three groups. In Group One, NSCs were cultured in DMED/F12 in which Schwann cells had grown for one day. In Group Two, NSCs and Schwann cells were co-cultured. In Group Three, NSCs were cultured in DMEM/F12. The morphology of NSCs was checked and beta-tubulin, GalC, hoechst 33342 and GFAP labellings were detected. RESULTS: In Group One, all neural spheres were attached to the bottom and differentiated. The majority of them were beta-tubulin positive while a few of cells were GFAP or GalC positive. In Group Two, neural spheres remained undifferentiated and their proliferation was inhibited in places where Schwann cells were robust. In places where there were few Schwann cells, NSCs performed in a similar manner as in Group One. In Group Three, the cell growth state deteriorated day after day. On the 7th day, most NSCs died. CONCLUSION: The secretion of rat Schwann cells has a growth supportive and differentiation-inducing effect on human NSCs. |
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| Keywords: | Stem cells Schwann cells Nerve growth factors Coculture Immunohistochemistry |
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