血小板激活因子和维甲酸对中性粒细胞功能和ICAM－1，ELAM－1表达的影响

目的：探讨血小板激活因子（ＰＡＦ）和维甲酸（ＲＡ）对多形核中性粒细胞（ＰＭＮ）功能和肺微血管内皮细胞（ＰＭＶＥＣ）ＩＣＡＭ－１和Ｅ－选择素（ＥＬＡＭ－１）表达的影响。方法：采用作者创建的无创性分离法分离培养ＰＭＶＥＣ；将ＰＡＦ和ＲＡ分别加入培养有ＰＭＶＥＣ的９６孔板中，观察二者对ＰＭＮ－ＰＭＶＥＣ粘附的影响；ＥＬＩＳＡ法测定ＰＭＶＥＣ上ＥＬＡＭ－１和ＩＣＡＭ－１的表达；测定ＰＡＦ和ＲＡ对ＰＭＮ的趋化性、聚集和酸性磷酸酶的释放的影响。结果：（ｌ）ＰＭＶＥＣ用ＰＡＦ处理３．５ｈ后，它与ＰＭＮ的粘附率从５７．３％增加到７２．８％（Ｐ＜０．０１）。而用ＰＡＦ处理ＰＭＮ同样可增加ＰＭＮ－ＰＭＶＥＣ的粘附率。（２）ＲＡ可阻断未刺激ＰＭＮ与ＰＡＦ刺激的ＰＭＶＥＣ之间的粘附，但对ＰＡＦ刺激的ＰＭＮ与未刺激的ＰＭＶＥＣ之间的粘附却无作用。（３）ＰＡＦ可增加ＰＭＶＥＣ上ＩＣＡＭ－ｌ和ＥＬＡＭ－ｌ的表达、增加酵母多糖激活血清和组胺对ＰＭＮ的趋化性、增加ＰＭＮ的聚集和ＰＭＮ酸性磷酸酶的释放。（４）ＲＡ则可抑制ＰＡＦ诱导的ＰＭＮ聚集、酸性磷酸酶的释放及ＰＭＮ对酵母多糖刺激血清和组胺的趋化性，而ＩＣＡＭ－１和ＥＬＡＭ－１的表达却未改变

The effects of platelet-activating factor and retinoic acid on the expression of ELAM-1 and ICAM-1 and the function of neutrophils

To investigate the effects of platelet-activating factor(PAF) and retinoic acid(RA) on the expression of ELAM-1 and ICAM-1 and the function of neutrophils. Methods: Pulmonary microvascular endothelial cells(PMVECs) were isolated by the method founded by the author himself. PAF and RA were added to PMVECs monolayers grown to confluency on 96 microplates. Then the effects of both on PMN-PMVECs adherence were measured. The expression of ICAM-1 and ELAM-1 on PMVECs were determined by using ELISA. We also determined the changes of PMNs chemotaxis to zymosan-activated serum and histamine, and PMNs aggregation and the release of acid phosphatase from PMNs when treated by PAF and RA. Results: (1)Preincubation of PMNs with PAF for 3. 5h increased the adhesion rate of polymorphonuclear leukocytes ( PMNs) to PMVECs from 57. 3% to 72. 8%(P<0. 01). Preincubation of PMNs with PAF also increased PMN-PMVEC adhesion rate. (2)All-trans retinoic acid blocked the adherence of untreated PMNs to PAF-pretreated PMVECs but not the adherence of PAF-pretreated PMNs to untreated PMVECs. (3) PAF increased the expression of intercellular adhesion molecule-1(ICAM-1) and E-selection(ELAM-1) on PMVECs, PMNs chemotaxis to zymosan-activated serum and histamine, and PMNs aggregation and the release of acid phosphatase from PMNs. (4) Co-incubation of RA inhibited PAF-induced PMN aggregation, the release of acid phosphatase from PMNs, and PMN chemotaxis to zymosan- activated serum and histamine while the expression of ICAM-1 and ELAM-1 did not change. Conclusion: PAF can activate PMNs,increase the expression of the cellular adhesion molecules on PMVECs and promote the adherence of PMN-PMVEC. RA can be used to ameliorate PMN-mediated infiammation.