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Co-expression of mouse and rat haemoglobins in interspecific hybrids of mouse and rat erythroleukemia cells |
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| Authors: | Irene Greiser-Wilke Gerhard Steinheider Eberhard Jentsch Donna Arndt-Jovin Norbert Kluge Hans J. Eibl Wolfram Ostertag |
| Affiliation: | 1. Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 63-73, D-1000 Berlin 33, F.R.G.;2. Institut für Molekularbiologie and Biochemie der Freien Universität Berlin, Arnimallee 22, D-1000 Berlin 33, F.R.G.;3. Kinderklinik der Universität Göttingen, Hermann-Rein-Straβe, D-3400 Göttingen, F.R.G.;4. Max-Planck-Institut für Biophysikalische Chemie, Am Faβberg, D-3400 Göttingen, F.R.G.;5. Heinrich-Pette-Institut, Universität Hamburg, D-2000 Hamburg 20, F.R.G. |
| Abstract: | A modified technique for cell fusion with lysolecithin-lipid emulsions was used to generate hybrid erythroleukemia cell lines from Friend leukemia mouse cells (FLC) and chemically transformed rat erythroleukemia cells. Chromosome analysis of the hybrid cells showed the presence of both parental genomes even after long culture periods. The hybrids were still able to undergo erythroid differentiation after dimethylsulphoxide (DMSO) stimulation. Analysis of the globin chains from the DMSO-stimulated cells showed that both the rat and the mouse erythroid phenotypes were expressed. This demonstrates the compatibility of the regulatory genetic elements for the control of erythroid differentiation in cell hybrids of erythroleukemic populations from different species. |
| Keywords: | Cell fusion erythroleukemia Friend leukemia cells haemoglobin co-expression carboxymethyl-cellulose dimethylsulphoxide Friend leukemia cells hypoxanthin aminopterin thymidine hypoxanthine-guanine-phosphoribosyltransferase (E.C. 2.4.2.8) thymidinekinase (E.C. 2.7.1.21) |
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