Probing human malignant T cells with lectins: A comparison with their surface antigen patterns defined by monoclonal antibodies |
| |
Authors: | Yolande Richard Laurence Boumsell Jean Lemerle Francoise Valensi Alain Bernard |
| |
Affiliation: | 1. Laboratoire d''Immunologie des Tumeurs de l''Enfant, Institut Gustave-Roussy, 94800 Villejuif, France;2. INSERM U 93, 75010 Paris, France |
| |
Abstract: | Tumor cells from 40 children and 13 adults with T cell malignancies were assessed for staining with fluorescinated peanut agglutinin (PNA) and soybean agglutinin (SBA). These cell populations had also been characterized for surface antigens using a series of monoclonal antibodies (Mo. Ab.) that permit an assignment of malignant cells to discrete stages of normal T cell differentiation. We had previously shown a clear correspondence between lectin- and Mo. Ab.-defined cell compartments within thymuses from normal children and T cells in peripheral blood. We report here that the pattern of reactivity of malignant T cells populations with lectins correlates closely the degree of maturation, as assessed by Mo. Ab. Thus, utilization of lectins together with Mo. Ab., can be clinically useful to characterize T cell malignancies. This observation shows that, in spite of a high degree of heterogeneity of malignant T cell populations from one patient to the other, in their pattern of surface antigens, these populations seem essentially to conform to the scheme of normal T cell differentiation. |
| |
Keywords: | monoclonal antibodies peanut agglutinin soybean agglutinin acute lymphoblastic leukemia lymphoblastic lymphoma chronic lymphocytic leukemia |
本文献已被 ScienceDirect 等数据库收录! |
|