Stimulation of haemopoietic stem cell proliferation: Characteristics of the stimulator-producing cells |
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| Authors: | Eric G. Wright Abdul M. Ali Andrew C. Riches Brian I. Lord |
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| Affiliation: | 1. Department of Anatomy and Experimental Pathology, University of St. Andrews, St. Andrews, Fife KY16 9TS, U.K.;1. Paterson Laboratories, Manchester, U.K. |
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| Abstract: | Media conditioned by regenerating murine bone marrow cells contain a stimulator of haemopoietic stem cell proliferation. Fractionated cell populations have been examined for production of this stimulatory activity in order to characterize its cellular source. The stimulator is produced by adherent, phagocytic radioresistant, Thy 1.2?, Fc+ cells in a population concentrated in a density range of 1.064 – 1.072 g/ml. The results indicate that the producer cells reside in the heterogenous mononuclear phagocytic population of the bone marrow. |
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| Keywords: | Stem cell proliferation regulation microenvironment macrophages spleen colony-forming unit (haemopoietic stem cells) regenerating bone marrow (cells obtained 5–7 days after 4.5 Gy irradiation) conditioned medium 30,000–50,000 mol. wt range fraction of CM obtained by Amicon Diaflo ultrafiltration phenylhydrazine (used as a prefix denotes cells taken on day 7 from mice injected with 60 mg/kg s.c. on days 0, 1 and 3) |
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