鼠bcl-XL基因在CHO细胞中的表达 |
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引用本文: | 王贤辉,梁米芳,李德新,徐静,米力,陈志南. 鼠bcl-XL基因在CHO细胞中的表达[J]. 医学争鸣, 2003, 24(24): 2217-2219 |
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作者姓名: | 王贤辉 梁米芳 李德新 徐静 米力 陈志南 |
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作者单位: | 1. 第四军医大学细胞工程中心,陕西,西安,710033 2. 中国预防医学科学院病毒学研究所 |
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基金项目: | 动物细胞大规模高效培养中试技术平台的建立(2 0 0 2AA2Z344A) |
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摘 要: | 目的:构建携带鼠bcl-XL基因的真核表达载体,并将其在CHO细胞中表达。方法:用反转录PCR获取鼠bcl-XL全长cDNA序列,将其克隆进pEGFP重组融合表达质粒,脂质体法转染CHO细胞,荧光检测目的蛋白表达。结果:成功获得了鼠bcl-XL cDNA,构建了编码鼠bcl-XL的真核融合表达载体pEGFP-bcl-XL,转染CHO细胞后观察到融合蛋白表达。结论:鼠bcl-XL基因的克隆及融合表达,为进一步在高密度、大规模细胞培养中利用bcl-XL具有重要意义。
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关 键 词: | 基因 bcl-XL CHO细胞 基因表达 |
文章编号: | 1000-2790(2003)24-2217-03 |
修稿时间: | 2003-08-10 |
Expression of murine bcl-XL gene in CHO cells |
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Abstract: | AIM: To construct eukaryotic expression vector containing murine bcl X L and EGFP fusion gene and to express it in CHO cells. METHODS: RT PCR was used to obtain murine bcl X L cDNA. Then the recombinant expression vector pEGFP bcl X L was constructed by cloning bcl X L cDNA into eukaryotic expression vector pEGFP. After transfection into CHO cells through lipofectamine 2000 TM , the expression of fusion gene was detected by fluoroscopy. RESULTS: The obtained murine bcl X L cDNA was consistent with that in GenBank. The eukaryotic expression vector pEGFP bcl X L was successfully constructed and expressed in CHO cells. CONCLUSION: The cloning and expression of murine bcl X L gene are of significance in further use of bcl X L gene in high density large scale cell culture. |
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Keywords: | genes bcl X L CHO cells gene expression |
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