| APOPTOSIS OF TUMOR CELLS IN LECTIN－DEPENDENTLYMPHOKINE－ACTIVATED KILLER CELLMEDIATED CYTOTOXICITY |
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| 引用本文： | 董海东,邢嵘,郭连英,杨翠红,钱振超.APOPTOSIS OF TUMOR CELLS IN LECTIN－DEPENDENTLYMPHOKINE－ACTIVATED KILLER CELLMEDIATED CYTOTOXICITY[J].中国癌症研究,1996,8(1):14-18. |
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| 作者姓名： | 董海东 邢嵘 郭连英 杨翠红 钱振超 |
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| 作者单位： | Dong Haidong; Xing Rong; Guo Lianying;Yang Cuihong; Qian Zhenchao;( T Cell Laboratory，Department of Immunology，Beijing Medical University，Beijing 100083) (Department of Pathophysiology，Dalian Medical University，Dalian 116023) |
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| 摘 要： | ＡＰＯＰＴＯＳＩＳＯＦＴＵＭＯＲＣＥＬＬＳＩＮＬＥＣＴＩＮ－ＤＥＰＥＮＤＥＮＴＬＹＭＰＨＯＫＩＮＥ－ＡＣＴＩＶＡＴＥＤＫＩＬＬＥＲＣＥＬＬＭＥＤＩＡＴＥＤＣＹＴＯＴＯＸＩＣＩＴＹＤｏｎｇＨａｉｄｏｎｇ董海东；ＸｉｎｇＲｏｎｇ邢嵘；ＧｕｏＬｉａｎｙｉｎ...
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| 修稿时间： | 29 December 1995 |
Apoptosis of tumor cells in lectin-dependent lymphokine-activated killer cell-mediated cytotoxicity |
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| Dong Haidong,Xing Rong,Guo Lianying,Yang Cuihong,Qian Zhenchao.Apoptosis of tumor cells in lectin-dependent lymphokine-activated killer cell-mediated cytotoxicity[J].Chinese Journal of Cancer Research,1996,8(1):14-18. |
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| Authors: | Dong Haidong Xing Rong Guo Lianying Yang Cuihong Qian Zhenchao |
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| Institution: | (1) T Cell Laboratory, Department of Immunology, Beijing Medical University, 100083 Beijing;(2) Department of Pathophysiology, Dalian Medical University, 116023 Dalian |
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| Abstract: | By using DNA electrophoresis and propidium iodide(PI) staining flow cytometry(FACS) analysis, we studied the mechanisms of lymphokine-activated killer(LAK) cell-mediated cytotoxicity. In the presence of pokeweed mitogen(PWM), human LAK cells induced DNA fragmentation of two leukemic cell lines(U937 cells and Raji cells) and two solid tumor cell lines(SW1116 cells and Hep-2 cells), a hallmark of apoptosis. The reactions were carried out at the effector/target ratio of 1:1 in 4h co-culture. Pretreatment with RNA and protein synthesis inhibitors(actinomycin D and cyclo hexamide) did not prevent the target cells from apoptosis.As the TNF-resistant tumor cell lines such as SW1116 cells and Raji cells were also triggered to apoptosis, other factors than TNF may play the role. DNA-PI staining FACS analysis also suggested that a part of LAK cells underwent apoptosis to some extent during incubation with target cells. The results provide a new way to investigate the mechanisms of the cytotoxicity of LAK cells, and a new possibility to enhance the efficiency of adoptive tumor therapy with LAK cells. |
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| Keywords: | Lectin LAK cells Apoptosis |
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