| 尿路致病性大肠埃希菌Ⅰ型菌毛fimH基因核酸疫苗免疫效果评价 |
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| 引用本文： | 耿丽,何深一,张玉梅,李波清,柏雪莲.尿路致病性大肠埃希菌Ⅰ型菌毛fimH基因核酸疫苗免疫效果评价[J].中国病原生物学杂志,2011(3). |
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| 作者姓名： | 耿丽 何深一 张玉梅 李波清 柏雪莲 |
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| 作者单位： | 山东大学医学院寄生虫学教研室;滨州医学院病原生物学教研室; |
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| 摘 要： | 目的研究尿路致病性大肠埃希菌Ⅰ型菌毛fimH基因核酸疫苗产生的免疫反应及其对小鼠的免疫保护作用,并比较不同免疫途径产生的免疫效果。方法选取BALB/c小鼠40只,随机分为4组(PBS组、空质粒组、pcD-NA3.0-fimH肌肉注射组、pcDNA3.0-fimH滴鼻组),用构建的fimH基因真核表达载体pcDNA3.0重组质粒分别通过肌肉注射和滴鼻(粘膜)免疫BALB/c小鼠,同时分别以载体质粒(pcDNA3.0)和PBS液为空质粒对照和空白对照,经股四头肌注射免疫小鼠,于第3周和第5周加强免疫。每次免疫前及末次免疫后2周采血检测特异IgG抗体。末次免疫后第10 d,以UPEC分离株菌液进行尿道上行攻击,攻击后第5 d进行尿液细菌培养和计数。结果免疫后,肌注组与滴鼻组小鼠血清特异性IgG抗体水平与对照组及空质粒组比较显著升高(P<0.05);UPEC分离株菌液攻击小鼠后,pcDNA3.0-fimH肌注组与滴鼻组小鼠尿液菌落数较对照组及空质粒组显著减少(P<0.05)。结论 pcDNA3.0-fimH基因疫苗可诱导BALB/c小鼠产生特异性体液免疫,对小鼠尿道上行感染具有一定的免疫保护作用,且不同的免疫途径免疫...
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| 关 键 词： | 尿路致病性大肠埃希菌 Ⅰ型菌毛 fimH基因 免疫效果 |
Evaluation of the immunoprotection from type 1 pili from uropathogenic Escherichia coli provided by a fimH vaccine |
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| GENG Li,HE Shen-yi,ZHANG Yu-mei,LI Bo-qing,BAI Xue-lian.Evaluation of the immunoprotection from type 1 pili from uropathogenic Escherichia coli provided by a fimH vaccine[J].Journal of Pathogen Biology,2011(3). |
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| Authors: | GENG Li HE Shen-yi ZHANG Yu-mei LI Bo-qing BAI Xue-lian |
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| Institution: | GENG Li1,HE Shen-yi1,ZHANG Yu-mei2,LI Bo-qing2,BAI Xue-lian2 (1.Department of Parasitology,School of Medicine,Shandong University,Jinan 250012,China,2.Department of Pathogen Biology of Binzhou Medical College,Yantai 264003,Shandong,China) |
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| Abstract: | Objective To investigate the immunoreaction of type 1 pili from uropathogenic Escherichia coli to a FimH vaccine and its immune protection in mice and to compare immunoprotection provided by different routes of immunization.Methods A total of 40 BALB/c mice were selected and randomly divided into 4 groups: a PBS group,a pcDNA3.0 group,an experimental group receiving intramuscular injection,and an experimental group receiving intranasal immunization.Recombinant pcDNA3.0-FimH was used to immunize BALB/c mice ... |
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| Keywords: | UPEC Type 1 pilus fimH gene immunoprotection |
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