铭铂诱导人卵巢癌细胞A2780凋亡

目的：观察铭铂诱导的人卵巢癌细胞A2780凋亡，探讨其抗肿瘤作用机理。方法：用MTT法测定了铭铂对A2780的细胞毒作用；荧光显微镜从形态学上观察凋亡发生，琼脂糖DNA电泳检测程序性细胞死亡特征的DNA降解。结果：铭铂对A2780细胞有细胞毒作用，半数抑制浓度具有药物接触细胞时间依赖性；铭铂处理的A2780细胞形态学上显示出凋亡细胞特性，琼脂糖凝胶电泳显示出特征性程序性细胞死亡DNA梯形图谱。结论：铭铂能够诱导A2780细胞凋亡，诱导肿瘤细胞凋亡是其一个抗肿瘤作用机理。

Apoptosis of Human Ovarian Carcinoma Cell Line A2780 Induced by Minoplatin

Objective:To study the anticancer mechanism of minoplatin by observing the induction of apoptosis.Methods:Cytotoxicity of minoplatin was tested by MTT method.Morphological changes of apoptosic cells were investigated by fluorescence microscope.DNA fragmentation was analysed by agarose gel electrophoresis.Results:Minoplatin has distinct cytotoxicity to human ovarian cancer cells,and IC 50 (i e the concentration that reduce the absorbance value to 50% of the control) was related to the time of the drug exposed to cells.Minoplatin-treated ovarian cells showed morphological characteristics of apoptosis.A DNA ladder fragmentation was demonstrated on agarose gel electrophoresis.Conclusion:Minoplatin can induce A2780 cell to apoptosis,and one mechanism of its anticancer effects is to trigger apoptosis.