HPLC法测定复方麝香草酚滴耳液中两种组份的含量

目的建立同时测定复方麝香草酚滴耳液中麝香草酚和水杨酸含量的方法。方法采用高效液相色谱法。色谱柱为Shim-pack VP-ODS(4.6×150mm,5μm),流动相为甲醇-水-磷酸(60∶40∶0.3),检测波长220nm,柱温30℃。结果水杨酸和麝香草酚分别在0.12~0.28mg.mL-1和0.06~0.14 mg.mL-1范围内线性关系良好,平均回收率分别为101.2%(RSD=0.57%)和100.1%(RSD=0.64%)。结论该方法可用于复方麝香草酚滴耳液的质量控制。

Simultaneous determination of two components in complex thymol ear drops by HPLC

OBJECTIVE To establish a method for the determination of thymol and salicylic acid in complex thymol ear drops.METHODS The column was shim-pack VP-ODS(4.6×150mm,5μm),the mobile phase consisted of methanol-water-phosphoric acid(60∶40∶0.3),the detection wavelength was 220nm,the column temperature was 30℃.RESULTS There was a good linear relationship when the content of salicylic acid and thymol was in the range of 0.12～0.28mg·m-1,and 0.06～0.14 mg·mL-1respectively.The average recoveries of salicylic acid and thymol were 101.2%(RSD=0.57%) and 100.1%(RSD=0.64%),respectively.CONCLUSION The method Can be applied to the quality control of complex thymol ear drops.