| 氯地滴眼液中氯霉素的含量测定 |
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| 引用本文： | 张黎明,杨友兰,蔡长坤.氯地滴眼液中氯霉素的含量测定[J].基层医学论坛,2008,12(7):193-195. |
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| 作者姓名： | 张黎明 杨友兰 蔡长坤 |
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| 作者单位： | 上海市第十人民医院,上海,200072 |
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| 摘 要： | 目的建立一阶导数分光光度法测定氯地滴眼液中氯霉素的含量。方法一阶导数分光光度法，在305nm处测定氯地滴眼液的振幅D。结果线性范围为20．13-30，01μg／ml，r=0．99989（n=5）；平均回收率为99，78％，RSD=0．227％（n=5），氯地滴眼液中地塞米松及辅料无影响。结论该法操作简便、准确，消除了地塞米松的影响，适合于医院制剂氯地滴眼液中氯霉素的含量测定。
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| 关 键 词： | 氯霉素 一阶导数分光光度法 含量测定 氯霉素地塞米松滴眼液 |
Determination of content of chloramphenicol in chloramphenicol and dexamethasone eye drops |
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| Zhang Liming,Yang Youlan,Cai Changkun.Determination of content of chloramphenicol in chloramphenicol and dexamethasone eye drops[J].Public Medical Forum Magazine,2008,12(7):193-195. |
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| Authors: | Zhang Liming Yang Youlan Cai Changkun |
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| Institution: | Zhang Liming,Yang Youlan,Cai Changkun. Department of Pharmacy,Shanghai Tenth People's Hospital,Shanghai 200072,China |
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| Abstract: | Objective To establish a method for determination of content of chloramphenicol in chloramphenicol and dexamethasone eye drops by primary derivative spectrophotometry.Methods Using primary derivative spectrophotometry,the amplitude D of chloramphenicol and dexamethasone eye drops was determinated at the wavelength of 305 nm. Results linearity of chloramphenicol content was revealed over the range of 20.13-30.01 ug/ml,r=0.99989(n=5).The average recovery was 99.78% with RSD of 0.227%(n=5). Primary derivative spectrophotometry could eliminate the interference of dexamethasone and excipient.Conclusion It was a rapid,convenient and accurate method and eliminate the interference of dexamethasone.It could be fit for the determination of content of chloramphenicol in chloramphenicol and dexamethasone eye drops in hospital. |
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| Keywords: | Chloramphenicol Primary derivative spectrophotometry Content determination Chloramphenicol and dexamethasone eye drops |
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