The production of inactive phage P22 particles following induction |
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| Authors: | V Israel |
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| Affiliation: | 1. Department of Human Genetics, University of Michigan, 1133 E. Catherine St., Ann Arbor, Michigan 48104 USA;2. The Lawrence D. Buhl Center for Human Genetics, University of Michigan, 1133 E. Catherine St., Ann Arbor, Michigan 48104 USA;1. West Pomeranian University of Technology, Szczecin, Faculty of Chemical Technology and Engineering, Department of Chemical and Process Engineering, Piastów Avenue 42, 71-065, Szczecin, Poland;2. Pomeranian Medical University in Szczecin, Chair of Microbiology, Immunology and Laboratory Medicine, Department of Laboratory Medicine, Powstańców Wielkopolskich Avenue 72, 70-111, Szczecin, Poland;1. Ankara University Faculty of Pharmacy, Department of Pharmaceutical Microbiology, 06100, Ankara, Turkey;2. Zonguldak Bulent Ecevit University, Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Zonguldak, Turkey;3. Zonguldak Bulent Ecevit University, Faculty of Medicine, Department of Medical Biology, Zonguldak, Turkey |
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| Abstract: | Using an in vitro system for tail attachment, it is demonstrated that following ultraviolet (UV) induction of Salmonella typhimurium LT2 (P22) most of the particles formed lack a functional tail. This effect is found at all temperatures studied, but is more pronounced at temperatures of 37° and above. Similar results are described for thermal and spontaneous induction. Tailless particles are found following infection only at temperatures of 42° and above. Temperature pulse experiments on UV-induced lysogens suggest that late in the growth cycle tail synthesis is inhibited by high temperature. There is also an earlier temperature-sensitive step, which probably causes the production of inactive heads at 40°. |
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