两个品系小鼠肺纤维化模型的比较观察

目的采用气管内注射博莱霉素（Bleomycin，BLM）的方法，比较观察两个品系小鼠肺纤维化程度，确定适用的肺纤维化小鼠模型。方法BALB／c小鼠和C57BL／6小鼠分别分成模型组和对照组，模型组气管内注射博莱霉素（5mg／kg），对照组气管内注射相同体积生理盐水。所有小鼠于造模后第28日处死，观察肺系数、肺组织病理学改变及羟脯氨酸含量的变化。结果光镜下各模型组肺组织呈不同程度纤维化，其中以C57BL／6小鼠最为明显；模型组的羟脯氨酸（HYP）含量均显著高于对照组；C57BL／6小鼠模型组肺系数及HYP明显高于BALB／c小鼠模型组。结论通过气管内注射博莱霉素，BALB／c及C57BL／6小鼠均能复制肺纤维化模型，以C57BL／6小鼠肺纤维化程度最高，获得的肺纤维化模型较为理想。

Comparative Observation on Pulmonary Fibrosis Models between Two Strains of Mice

Objective To provide a better animal model of pulmonary fibrosis by comparing the model establishment in different strains of mice. Methods BALB/c and C57BL/6 mice were divided into model and control group respectively, which received intratracheal injection of bleomycin （BLM） and normal saline, respectively. The mice were sacrificed 28 days after treatment. The masson mass and the degree of fibrosis in the left pulmonary lobe were observed by light microscope. Hydroxyproline contents in right pulmonary lobe were measured. Results The pulmonary consolidation and collagen deposition appeared in all bleomycin groups especially in the C57BL/6 group. Hydroxyproline contents in pulmonary tissue in C57BL/6 mice was the highest and normal saline group was the lowest one. Conclusion The pulmonary fibrosis model can be established in BALB/c and C57BL/6 mice by intratracheal injection of bleomycin, while the C57BL/6 mice was more suitable.