EGCG对Goto-kakizaki大鼠骨骼肌组织GLUT4表达的影响

目的 研究表没食子儿茶素没食子酸酯（EGCG）对自发性Ⅱ型糖尿病GK大鼠的作用及对骨骼肌组织葡萄糖转运蛋白4（GLUT4）表达的影响.方法 自发性2型糖尿病大鼠GK大鼠40只,同系健康对照Wistar大鼠10只,大鼠随机分为：正常对照组、Ⅱ型糖尿病组、Ⅱ型糖尿病低剂量EGCG（50 mg/kg）治疗组、中剂量（100 mg/kg）EGCG、高剂量EGCG（300 mg/kg）治疗组,6周后测血糖、血脂和骨骼肌GLUT4的水平.结果 EGCG可明显改善GK大鼠的OGTT及血脂,增加大鼠模型骨骼肌胞膜上GLUT4蛋白表达.结论 EGCG可降低TIIDM大鼠血糖水平,其机制与提高糖尿病大鼠骨骼肌中GLUT4表达有关.

The Effect of Epigalloca-techin-3-gallate(EGCG) on Expression of GLUT4 in Skeletal Muscle Tissue of Goto-kakizaki Rats

Objective To investigate the effect of epigalloca-techin-3-gallate（EGCG）on expression of GLUT4 in the skeletal muscle tissue of Goto-kakizaki rats. Methods Goto-kakizaki diabetic rats （GK, n=40） were observed and Wistar rat （n=i0） as control. Rats were randomly divided into five groups： normal control group, TIIDM model group, diabetic model plus EGCG of 50mg/kg, 100mg/kg and 300mg/kg therapy group .After 6 weeks,blood glucose,serum lipids and the expression of GLUT4 were detected. Results EGCG obviously improved the OGTT and serum lipids, and increased the expression of GLUT4 in skeletal muscle membrane fraction. Conclusion EGCG can decrease the levels of blood glucose in type II diabetic rats, and its mechanisms might be closely related to the elevation of the level of GLUT4 in the skeletalmuscle tissues of rats.