| 高效液相色谱法测定藏药结血蒿中熊果酸含量 |
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| 引用本文： | 顿珠,米玛,陈静,泽仁达瓦.高效液相色谱法测定藏药结血蒿中熊果酸含量[J].中国医药导报,2014(14):96-98,101. |
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| 作者姓名： | 顿珠 米玛 陈静 泽仁达瓦 |
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| 作者单位： | 西藏藏医学院,西藏拉萨850000 |
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| 基金项目： | 西藏自治区第二批科技计划项目（编号201210）. |
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| 摘 要： | 目的 建立藏药结血蒿药材中活性成分熊果酸的定量分析方法 ，测定熊果酸含量。方法 采用高效液相色谱法（HPLC法），色谱柱为Aglient XDB C18（4．6mm×150mm，5μm），流动相为甲醇-0．1％磷酸溶液，体积流量1．0mL/min，检测波长210nm。结果 熊果酸在60-190μg的质量浓度范围内与峰面积呈良好的线性关系（r=0．9997）：平均回收率为93．22％，RSD为2．64％。结论 所建立的HPLC法简便快捷、准确。可用于结血蒿药材成分的定量测定及质量控制，为安全、合理、有效用药及制定藏药结血蒿的药材质量标准提供科学依据。
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| 关 键 词： | 结血蒿 熊果酸 定量测定 质量控制 高效液相色谱法 |
Content determination of ursolic acid in Artemisia vestita Wall. ex Bess. by HPLC method |
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| Institution: | Dunzhu Mima, CHEN Jing (Zerendawa Tibetan Traditional Medical College, Tibet Autonomous Region, Lhasa 850000, China) |
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| Abstract: | Objective To establish a HPLC method for determining ursohc acid in different fractions of Artemisia vestita Wall, ex Bess. Methods The separation was performed on Aglient XDB C18 （4.6 mm× 150 mm, 5 μm） by HPLC, using methanol - 0.1% phosphoric as mobile phase. The flow rate was 1.0 mL/min and the detection wavelength was 210 nm. Results The linear range of ursolie acid was 50-190 μg （ r = 0.9997）. The average recoveries was 93.22% with RSD of 2.64%. Conclusion This method is simple, rapid and sensitive, and it can be used for the quality control of Artemisia vestita Wall. ex Bess |
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| Keywords: | Artemisia vestita Wall ex Bess Ursolic acid Assay Quality control HPLC |
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