Ang-1 和 Tie2 在高血压患者内皮祖细胞中表达水平及临床意义研究

目的探讨Ang-1及其受体Tie2在高血压患者循环内皮祖细胞中的表达水平并评价其临床意义。方法选择杭州师范大学附属医院2012年1月～2013年6月高血压I、Ⅱ级和Ⅲ级患者各15例：正常体检人群30名为对照组。lit—PCR法检测各组外周血中内皮祖细胞内Ang-1、Tie2mRNA表达水平；Western—blot法测定Tie2酪氨酸磷酸化水平。结果Ⅲ级高血压患者内皮祖细胞内Ang-1和Tie2mRNA表达水平均低于Ⅱ、I级高血压患者和对照组（P〈0．05），1I级高血压患者均低于I级高血压患者和对照组（P〈0．05）。Ⅲ级高血压患者内皮祖细胞Tie2磷酸化水平低于Ⅱ、I级高血压患者和对照组（P〈0．05），11级高血压患者Tie2磷酸化水平低于I级高血压患者和对照组（P〈0．05）。常规治疗后，内皮祖细胞Ang-1和Tie2mRNA表达及磷酸化Tie2水平均高于治疗前（P〈0．05）。结论患者循环内皮祖细胞内Ang-1和Tie2表达随高血压分级的增加逐渐下调．治疗后水平升高，可能与高血压病血管内皮细胞修复的调控机制密切相关。

Research on the expression level of Ang-1 and Tie2 of peripheral en- dothelial progenitor cells in patients with hypertension and its clinical sig- nificance

Objective To discuss the expression of Ang-1 and Tie2 of peripheral endothelial progenitor cells in pa- tients with hypertension, as well as its clinical significance. Methods 15 patients with grade I , II and grade Ill hyper- tension respectively were studied in the Affiliated Hospital of Hangzhou Normal University from January 2012 to June 2013. 30 healthy subjects were recruited as normal controls. Expression of Ang-1 and Tie2 mRNA of the peripheral blood endothelial progenitor cells were measured by RT-PCR, Tie2 tyrosine phosphorylation level was measured by Western-blot. Results mRNA levels of Ang-1 and Tie2 of endothelial progenitor cells in patients with grade llI hyper- tension were lower than those of the patients with grade ]1, I hypertension and control group （P 〈 0.05）, the levels of those in patients with grade II hypertension were lower than those of grade I hypertension and the control group （P 〈 0.05）. Tie2 tyrosine phosphorylation level of endothelial progenitor cells in patients with grade Ill hypertension was low- er than that of patients with grade H, grade I hypertension and control group （P 〈 0.05）, and that of grade II hyper- tension patients was lower than patients with grade I hypertension and the control group （P 〈 0.05）. After routine treatment, the expression of Ang-1 and Tie2 mRNA or Tie2 phosphorylation level were higher than those of before treatment （P 〈 0.05）. Conclusion Ang-1 and Tie2 expression of circulating endothelial progenitor cells in patients with hypertension reduced with the increasing of hypertension grade, but after treatment these levels elevated, may have par- ticipated in the regulation of vascular endothelial cells in patients with hypertension.