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中心组合设计-响应面分析法优选黄芩中黄芩苷的超声提取工艺
引用本文:马爽,赵岩,赵晓红,陆珞,蔡恩博,刘双利,张连学. 中心组合设计-响应面分析法优选黄芩中黄芩苷的超声提取工艺[J]. 中国医药导报, 2014, 0(9): 142-145
作者姓名:马爽  赵岩  赵晓红  陆珞  蔡恩博  刘双利  张连学
作者单位:[1]吉林农业大学,吉林长春130118 [2]吉林省食品药品审评中心,吉林长春130062
基金项目:国家科技支撑计划课题(编号2011BAI03B01);国家公益性行业科研专项课题(编号201303111);吉林省科技发展计划项目(编号20126046).
摘    要:目的 优化黄芩中黄芩苷的提取工艺.方法 采用中心组合设计方法,研究乙醇浓度、超声时间、液固比及其交互作用对黄芩苷提取率的影响,应用SAS软件和响应面分析相结合的方法,模拟得到回归方程的预测模型和可信度,并通过岭脊分析得到黄芩苷最佳提取条件.结果 黄芩苷最佳提取条件为:乙醇浓度67%、超声时间57 min、液固比150 mL/g,该条件下提取率为10.05%.结论 超声提取法是一种高效、快速提取黄芩中黄芩苷的方法.

关 键 词:黄芩  黄芩苷  中心组合设计  响应面分析  高效液相色谱法

Optimization of ultrasonic-assisted extraction of Baicalin from Scutellaria baicalensis Georgi using central composite design-response surface methodology
MA Shuang,ZHAO Yan,ZHAO Xiaohong,LU Luo,CAI Enbo,LIU Shuangli,ZHANG Lianxue. Optimization of ultrasonic-assisted extraction of Baicalin from Scutellaria baicalensis Georgi using central composite design-response surface methodology[J]. China Medical Herald, 2014, 0(9): 142-145
Authors:MA Shuang  ZHAO Yan  ZHAO Xiaohong  LU Luo  CAI Enbo  LIU Shuangli  ZHANG Lianxue
Affiliation:1.Jilin Agricultural University, Jilin Province, Changchun 130118, China; 2.Center for Food & Drug Evalution, Jilin Province, Changchun 130062, China;)
Abstract:Objective To optimize the extraction technique for Baicalin from Scutellaria baicalensis Georgi.Methods The effects of ethanol concentration,ultrasonic extraction time,the liquid-solid ratio and their interaction on extraction rate were studied by central composite design.The predictive model and reliability were developed by SAS software and response surface methodology.Results The optimal ultrasonic extraction conditions were achieved and listed as follows:ethanol concentration was 67%,ultrasonic extraction time was 57 min,the liquid-solid ratio was 150 mL/g,through ridge analysis,under the optimum conditions,the extraction yield of Baicalin was 10.05%.Conclusion Ultrasonic-assisted extraction is proved to be a kind of efficient and rapid method for extraction of baicalin from Scutellaria baicalensis Georgi.
Keywords:Scutellaria baicalensis Georgi  Baicalin  Central composite design  Response surface methodology  HPLC
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