高效液相色谱法测定氯霉素醇溶液中氯霉素的含量

目的研究高效液相色谱法测定氯霉素醇溶液中氯霉素的含量。方法采用高效液相色谱法，以EclipseXDB—C18（4．6mm×250mm，5μm）为色谱柱；流动相为0．01mol／L庚烷磺酸钠缓冲液（取磷酸二氢钾6．8g．用0．01mol／L庚烷磺酸钠溶液溶解并稀释至1000mL，再加三乙胺5mL，混匀，用0．01mol／L磷酸调节pH值至2．5）-甲醇（68：32）；流速1．0mL／min，检测波长277nm，进样量10μL。结果氯霉素在60—160μg／mL范围内与峰面积呈良好的线性关系，回归方程为A=17．79C＋17．13，r=0．9995；平均回收率为100．24％，RSD=1．26％。结论本方法简便快速，定量准确，结果稳定、重现性好，可用于氯霉素醇溶液中氯霉素的含量测定。

Determination of Chloramphenicol in Chloramphenicol Alcoholic Solution by HPLC

Objective To establish a method for the determination of Chloramphenicol in Chloramphenicol Alcoholic Solution by HPLC. Methods HPLC was used for quantitative analysis. Eclipse XDB-C18 （4.6 mm×250 mm, 5 μm） was used, 0.01 mol/L heptane sodium sulfonate buffer solution （1000 mL 0.01 mol/L heptane sodium sulfonate buffer solu- tion consisted of 6.8 g dipotassium phosphate, 5 mL triethylamine and 0.01 mol/L heptanes sulfonic acid sodium solution, adjusted pH to 2.5 by phosphoric acid solution） - methanol （68：32） was used as mobile phases, the flow rate was 1.0 mlJmin, the detective wavelength was at 277 nm and the injection volume was 10 μL. Results The calibration curve was linear in the range of 60-160 μg/mL for Cholramphenicol, and the equation of linear regression was A = 17.79C＋17.13 （r = 0.9995）; the average recovery rate was 100.24% and RSD was 1.26%. Conclusion The method is simple, accurate with good reproducibility and stability, and can be used for the quality control of the Chloramphenicol Alcoholic Solution.