牛血清白蛋白色谱柱的制备及对阿司匹林的含量测定

目的制备生物色谱柱，用于分离或测定药物成分。方法采用3-氨基丙基三甲氧基硅烷（APTS）与硅胶反应生成氨基功能化的活化硅胶，并与牛血清蛋白（BSA）共价结合形成BSA键合固定相，匀浆装柱制备成BSA生物色谱柱，以阿司匹林（Aspirin，ASP）为分析对象考察BSA固定相的结合性能和分离性能。结果BSA结合率为3．69μmol／g，ASP在BSA键合固定相色谱柱上的最大结合量为74mg／g，经BSA色谱柱测得阿司匹林肠溶片的含量为14．65μg／片，标示量为107．8％。结论这表明BSA与硅胶载体成功结合形成BSA键合固定相，所制备的BSA生物色谱柱具有良好的结合性能与分离性能。

Preparation of bovine serum albumin biological chromatographic column and its application in determination of Aspirin

Objective To prepare biological chromatographic column to separate and determine the ingredients of drugs. Methods Silica gel was reacted with 3-aminopropyl trimethoxylsilane to form amine functionalized activated silica gel, which was covalent binding with BSA to synthesize BSA bonded stationary phase. Then BSA bonded stationary phase was packed column with homogenization, thus BSA biological chromatographic column was prepared. Aspirin was taken as a detective object to examine the combination performance and separation performance of BSA bonded stationary phase. Results It came to that, the combined rate was 3.69 μmol/g, the biological chromatographic column was 74 mg/g, the amount of Aspirin in per tablet was 14.65 μg and the labeled amount was 107.8% detected by BSA biological chromatographic column. Conclusion All of above shows that BSA bonded stationary phase is synthesized successfully, and the BSA biological chromatographic column has good combination property and separation property.