载神经生长因子纳米柔性脂质体透血-脑屏障的研究

目的研究纳米柔性脂质体对促进神经生长因子（NGF）突破血脑屏障的作用。方法采用薄膜-超声法制备载NGF纳米柔性脂质体，免疫荧光法观察纳米柔性脂质体对NGF进入星形胶质细胞和血管内皮细胞的影响。根据实验要求分为对照组、游离NGF组、载NGF纳米柔性脂质体组。结果载NGF纳米柔性脂质体平均粒径为（83．1±1．7）nm，包封率为（64．2±2．8）％；对于星形胶质细胞和血管内皮细胞，游离NGF组细胞的NGF含量与对照组比较差异均无统计学意义（P〉0．05），而载NGF纳米柔性脂质体组[（33．52±6．45）、（27．26±4．08）]则显著高于对照组[（8．31±2．15）、（0．00±0．00）]（P〈0．05）。结论载NGF纳米柔性脂质体具有较高的包封率，纳米柔性脂质体可能成为蛋白质类大分子药物有效突破血脑屏障的载体。

Study on flexible nano-liposome loaded with nerve growth factor pene- trating blood-brain barrier

Objective To study the effect of flexible nano-liposome （FNL） on enhancing the penetration of nerve growth factor （NGF） penetrating blood-brain barrier. Methods The FNL loaded with NGF was prepared by the method of film- ing and uhrasounding. The effect of FNL on enhancing penetration of NGF to horizontal cell and vascular endothelial cell was observed through immumofluorescence method. According to the experimental requirements, they were divided into control group, free NGF group, flexible nano-liposome containing NGF group. Results The mean diameter and en- velope rate of FNLs loaded with NGF were （83.1±1.7） nm and （64.2±2.8）%, respectively. As for horizontal cell and vas- cular endothelial cell, the NGF contents of the free NGF group had no significant differences compared with control group （P 〉 0.05）, while flexible nano-liposome containing NGF group [（33.52±6.45）, （27.26±4.08）] were higher than those of control group [（8.31±2.15）, （0.00±0.00）] （P 〈 0.05）. Conclusion The FNL loaded with NGF possesses higher envelope rate, and FNL may be an effective vector for drugs with giant molecule to penetrate blood-brain barrier.