辛伐他汀对2型糖尿病患者内皮祖细胞增殖的影响及其机制探讨

目的观察辛伐他汀对2型糖尿病患者外周血来源内皮祖细胞（EPCs）增殖的影响，并初步探讨其机制。方法2型糖尿病合并高脂血症患者20例，随机分为辛伐他汀组（给予辛伐他汀20mg/d口服）和对照组（给予安慰剂）。治疗前及治疗后2、4周取外周血获取单个核细胞，培养7d后对获得的细胞进行分析，比较各组EPCs数目，并对血清中低密度脂蛋白-胆固醇（LDL-C）浓度与EPCs数目的改变进行相关分析。在EPCs培养体系中分别加入辛伐他汀、磷脂酰肌醇-3激酶，蛋白激酶通路（P13K／Akt）信号转导特异性抑制荆Ly294002。观察对其增殖的影响。结果辛伐他汀组患者治疗后2、4周外周血中EPCs明显增多（P〈0．01），其变化与LDL-C变化无相关性（P〉0．05）。体外实验证明辛伐他汀能促进EPCs的增殖，这种作用能被Ly294002阻断。结论辛伐他汀能促进EPC8的增殖，这种作用可能与激活P13K／Akt信号转导通路有关。

Effect of simvastatin on endothelial progenitor cells of patients with type 2 diabetic mellitus and its possible role

Objective To observe the effect of simvastatin on endothelial progenitor cells(EPCs) of patients with type 2 diabetic mellitus, and try to elucidate its possible role. Methods Twenty patients with type 2 diabetic mellitus and hyperlipidemia were randomly divided into 2 groups. Ten patients in group A were given simvastatin 20 mg/d, while 10 patients in group B were given placebo. Peripheral blood mononuclear cells were obtained before and 2, 4 weeks after the treatment, respectively. EPCs were cultured for 7 days. The numbers of EPCs in various time points in the two groups were counted. Linear correlation assays were used between the changes of concentration of LDL-C and numbers of EPCs. Simvastatin and Ly294002 were added into the culture system of EPCs, respectively. Proliferative functions were observed after 24 hours. Results The numbers of EPCs after treated with simvastatin in group A were obviously increased(P < 0.01), and showed no correlation with changes of LDL-C(P > 0.05). Simvastatin could improve the proliferative function of EPCs, while it could be blocked by Ly294002. Conclusion Simvastatin can increase the proliferation of EPCs and this kind of effect may be related with activating the PI3K/Akt signal pathway.