Ganoderma tsugae extract inhibits expression of epidermal growth factor receptor and angiogenesis in human epidermoid carcinoma cells: In vitro and in vivo |
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| Authors: | Shih-Chung Hsu Chien-Chih Ou Tzu-Chao Chuang Jhy-Wei Li Yi-Jen Lee Vinchi Wang Jah-Yao Liu Chin-Shiang Chen Song-Chow Lin Ming-Ching Kao |
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| Affiliation: | 1. Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan, ROC;2. Kang-Ning Junior College of Medical Care and Management, Taipei, Taiwan, ROC;3. Department of Obstetrics and Gynecology, Tri-Service General Hospital, Taipei, Taiwan, ROC;4. Department of Chemistry, Tamkang University, Tamsui, Taipei, Taiwan, ROC;5. Graduate Institute of Life Sciences, Tamkang University, Tamsui, Taipei, Taiwan, ROC;6. Graduate Institute of Pathology, National Defense Medical Center, Taipei, Taiwan, ROC;g Department of Pathology, Da-Chien General Hospital, Miaoli, Taiwan, ROC;h Department of Neurology, Cardinal Tien Hospital, Taipei, Taiwan, ROC;i School of Medicine, Fu-Jen Catholic University, Taipei, Taiwan, ROC;j Department of Horticulture and Biotechnology, Chinese Culture University, Taipei, Taiwan, ROC;k School of Biological Science and Technology, China Medical University, Taichung, Taiwan, ROC;l Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, ROC |
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| Abstract: | We examined the anti-angiogenic effects of Ganoderma tsugae methanol extract (GTME) on human epidermoid carcinoma A-431 cells. Our data indicate that GTME inhibits the expression of epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) in vitro and in vivo, and also inhibits the capillary tube formation of human umbilical vein endothelial cells (HUVECs). We also show that the suppression of VEGF expression by GTME can be restored by treatment with EGF. These results suggest that GTME inhibits VEGF expression via the suppression of EGFR expression, resulting in the downregulation of VEGF secretion from epidermoid carcinoma A-431 cells. These findings reveal a novel role for G. tsugae in inhibiting EGFR and VEGF expression, which are important for tumor angiogenesis and growth. Thus, GTME may provide a potential therapeutic approach for anti-tumor treatment. |
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| Keywords: | G. tsugae, Ganoderma tsugae GTME, Ganoderma tsugae methanol extract EGFR, epidermal growth factor receptor VEGF, vascular endothelial growth factor MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide HUVECs, human umbilical vein endothelial cells |
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