| 用PCR—SSCP方法探讨ras基因,p53基因突变与喉癌的关系 |
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| 引用本文： | 辛露,罗兵.用PCR—SSCP方法探讨ras基因,p53基因突变与喉癌的关系[J].山东医大基础医学院学报,2000,14(4):198-199. |
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| 作者姓名： | 辛露 罗兵 |
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| 作者单位： | [1]青岛市立医院耳鼻喉科 [2]青岛大学医学院微生物学教研室 |
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| 摘 要： | 目的：研究ｒａｓ基因、ｐ５３基因突变与喉鳞状细胞癌发病的关系。方法：应用多聚酶链反应－单链构像多态性分析（ＰＣＲ－ＳＳＣＰ）方法分析４０例喉鳞状细胞癌Ｈ－ｒａｓ，Ｋ－ｒａｓ，和Ｎ－ｒａｓ基因的第１２密码子，ｐ５３基因第５，７外显子中点突变的情况。结果：Ｈ－ｒａｓ基因突变９例，突变率为２２．５％（９／４０）；Ｋ－ｒａｓ基因突变８例，突变率为２０％（８／４０），Ｎ－ｒａｓ基因突变５例，突变率为１２．５
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| 关 键 词： | 基因P53 聚合酶链反应 喉肿瘤 PCR－SSCP |
The point mutation of ras and p53 genes in laryngeal squamous cell carcinomas |
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| Xin Lu,Zhao Shuyou,Luo Bing.The point mutation of ras and p53 genes in laryngeal squamous cell carcinomas[J].Journal of Preclinical Medicine College of Shandong Medical University,2000,14(4):198-199. |
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| Authors: | Xin Lu Zhao Shuyou Luo Bing |
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| Institution: | Xin Lu 1 Zhao Shuyou 1 Luo Bing 2 |
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| Abstract: | Objective:To evaluate the role of oncogene ras and suppressor gene p53 in laryngeal squamous cell carcinoma.Method:The point mutations of the 12th coden of ras gene and p53 E 5 and E 7 gene were studied in 40 cases with laryngeal squamous cell carcinomas by PCR SSCP(polymerase chain reaction single stranded conformational polymorphism).Result:22 out of 40(55%) primary tumors were detected the point mutations in the coden 12th of k ras and n ras,h ras.The point mutations of p53 E 5 and E 7 were found in 16 laryngeal tumors (16/40,40%).Conclusion:The point mutations of ras and p53 genes are both the important mechanisms of the occasion in laryngeal squamous cell carcinoma. |
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| Keywords: | Genes RAS Genes P53 Mutation Polymerase chain reaction Laryngeal neoplasms |
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