喉上皮性肿瘤与人乳头状瘤病毒的关系

目的：研究ＨＰＶ与喉上皮性肿瘤的关系。方法：采用一对ＨＰＶ Ｌ１区通用引物及６、１１、１６、１８型特异Ｅ６区引物多聚酶链反应，对１２８例喉癌及喉上皮增生性病变样本进行ＨＰＶ检测。结果：２４例检出ＨＰＶ，１６例为ＨＰＶ６型，４例ＨＰＶ１１例，１例ＨＰＶ１８型，２例未定型。６０例喉癌中５例阳性。其中３例６、１１、型均为整合状态，另２例为未定型。结论：大部分喉癌和上皮增生性病变与ＨＰＶ感染无关；良性型Ｈ

Relationship between laryngeal neoplasm and human papilloma virus

Objective:To investigate the relationship between the laryngeal neoplasm and human papilloma virus(HPV).Methods:The 128 cases with formalin fixed paraffin-imbedded,of laryngeal squamous cell carcinoma(LSCC) and laryngeal epithelium hyperplastic lesion(LEHL) were detected for HPV infection by PCR with HPV consensus L 1 primer and 6,11,16,18 type specific E 6 primers.Results:The presence of HPV DNA was detected only in 5 of 60 LSCC,11 of 15 laryngeal papillomas,4 of 10 dysplasia and 1 of 10 keratosis.None HPV was detected in 15 laryngeal polyps and 9 adjacent chronic laryngitis.Most of HPV found were benign types,except that one keratosis was HPV18.Conclusions:This study suggests that most of LSCC and LEHL are not associated with HPV infection.Benign HPV types also integrate with host cell genes in malignant lesions.Besides surgery,chemotherapy and radiation therapy,antiviral therapy should be accepted in a few HPV positive patients.