大孔树脂对川射干异黄酮的分离纯化工艺研究

 目的研究大孔吸附树脂分离纯化川射干中异黄酮的工艺条件及参数。方法以川射干异黄酮的吸附量和解析率为指标筛选大孔吸附树脂,并研究所选树脂分离纯化川射干异黄酮的吸附与解吸特性,优化纯化条件。结果DM-401型大孔树脂对川射干异黄酮的静态饱和吸附量为29.64 mg·g^-1,解吸率为91.54%;动态吸附量为27.30 mg·g^-1,解吸率为88.74%;上柱前粗提物中川射干异黄酮的含量为24.95%,经大孔树脂分离纯化后川射干异黄酮的含量为58.70%。结论DM-401型大孔树脂对川射干异黄酮有较好的吸附与解析特性,适合用于川射干异黄酮的分离与纯化。

Studies on Separation and Purification of Isoflavones from Chuanshegan by Macroporous Adsorption Resin

OBJECTIVE To optimize the purification method of the total isoflavones in Chuanshegan with macroporous adsorptive resin.METHODS The static adsorption/desorption characteristics were used as evaluation to select the better resin.The selected resin was studied focusing on the dynamic adsorption/desorption characteristics to optimize the purification conditions.RESULTS Resin DM-401 is selected to purify the total isoflavones in Chuanshegan.The static adsorption capacity/desorption ratio and the dynamic adsorption capacity/ desorption ratio for the isoflavones were 29.64 mg·g^-1/91.54% and 27.30 mg·g^-1/88.74%,respectively.The purity of the product before and after the resin purification process were 24.95% and 58.7%,respectively.CONCLUSION Macroporous adsorption resin DM-401 was suitable to purify the total isoflavones in Chuanshegan.