人苍白杆菌深圳分离株16S rRNA基因部分核苷酸序列分析

目的对深圳市布吉人民医院分离鉴定的1株人苍白杆菌进行16S rRNA基因的部分核苷酸序列分析,以探讨该菌株在分类学上的准确地位。方法对该菌株进行PCR扩增及16SrRNA基因序列测定,并与人苍白杆菌标准株及羊种布氏杆菌比较。结果3株细菌均可扩增到大小约900bp的16S rRNA基因片段,经核苷酸序列分析比较,发现人苍白杆菌深圳分离株与人苍白杆菌标准株和羊种布氏杆菌具有高度同源性,同源程度大于98%。结论该院分离的人苍白杆菌不仅与购自美国的人苍白杆菌ATCC株有高度同源性,而且与北京保存的羊种布氏菌在遗传学上也具有非常密切的亲缘关系。

Analysis of 16S rRNA gene partial nucleotides sequencing of an ochrobactrum anthropi strain isolated from Shenzhen

Objective To determine the taxonomic position of an O.anthropi strain isolated from our hospital.Methods The partial 16S rRNA gene of this strain was amplified by PCR and sequenced,compared with that of standard strains of O.anthropi and B.melitensis.Results A fragment about 900 bp of 16S rRNA gene could be amplified from each strain by PCR.The nucleotides sequencing analysis showed the DNA homology between the isolated strain and the standard strains of O.anthropi or B.melitensis were more than 98%, respectively.Conclusion The results of this research not only strengthen the phylogenic position of our isolated strain to be O.anthropi, but also in a close relationship with B.melitensis.